High Throughput Automated LC-MS/MS Analysis of Endogenous Small Molecule Biomarkers

被引：5

作者：

Grant, Russell P. ^{[1
]}

机构：

[1] Lab Corp Amer, Res & Dev, Burlington, NC 27215 USA

来源：

CLINICS IN LABORATORY MEDICINE | 2011年 / 31卷 / 03期

关键词：

Automation; Endogenous biomarkers; High throughput; LC-MS/MS; TANDEM MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; LIQUID-CHROMATOGRAPHY; METHYLMALONIC ACID; SAMPLE PREPARATION; TESTOSTERONE; DIAGNOSIS; PLASMA; SERUM; ASSAY;

D O I：

10.1016/j.cll.2011.07.008

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

This article describes the challenges and solutions that have been realized during the last 10 years by a number of clinical laboratories that have embraced automation and liquid chromatography-tandem mass spectrometry (LC-MS/MS) technologies for clinical utility. Current state-of-the-art liquid handling-based sample preparation, multiplexed chromatographic systems, and automated data review technologies are described, facilitating more than 2500 samples per system per day. Emerging technologies are discussed that may fundamentally drive the application of systems solutions for mass spectrometry to a broader clinical setting.

引用

页码：429 / +

页数：14

共 48 条

[1] Automated solid-phase extraction for concentration and clean-up of female steroid hormones prior to liquid chromatography-electrospray ionization-tandem mass spectrometry: An approach to lipidomics [J].

Alvarez Sanchez, B. ;

Priego Capote, F. ;

Ruiz Jimenez, J. ;

Luque de Castro, M. D. .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1207 (1-2) :46-54

[2] The impact of assay quality and reference ranges on clinical decision making in the diagnosis of androgen disorders [J].

Bhasin, Shalender ;

Zhang, Anqi ;

Coviello, Andrea ;

Jasuja, Ravi ;

Ulloor, Jagadish ;

Singh, Ravinder ;

Vesper, Hubert ;

Vasan, Ramachandran S. .

STEROIDS, 2008, 73 (13) :1311-1317

[3] A simple high-throughput method for the determination of plasma methylmalonic acid by liquid chromatography-tandem mass spectrometry [J].

Blom, Henk J. ;

van Rooij, Arno ;

Hogeveen, Marije .

CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2007, 45 (05) :645-650

[4] Two new techniques for sample preparation in bioanalysis: Microextraction in packed sorbent (MEPS) and use of a bonded monolith as sorbent for sample preparation in polypropylene tips for 96-well plates [J].

Blomberg, Lars G. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2009, 393 (03) :797-807

[5]

Bonfiglio R, 1999, RAPID COMMUN MASS SP, V13, P1175, DOI 10.1002/(SICI)1097-0231(19990630)13:12<1175::AID-RCM639>3.0.CO

[6]

2-0

[7] Mass spectrometry in the clinical laboratory [J].

Chace, DH .

CHEMICAL REVIEWS, 2001, 101 (02) :445-477

[8] Mass spectrometry in newborn and metabolic screening: historical perspective and future directions [J].

Chace, Donald H. .

JOURNAL OF MASS SPECTROMETRY, 2009, 44 (02) :163-170

[9]

CRAWFORD M, 2011, P 3 MASS SPECTR CLIN

[10] Plasma free Metanephrine measurement using automated online solid-phase extraction HPLC-Tandem mass Spectrometry [J].

de Jong, Wilhelmina H. A. ;

Graham, Kendon S. ;

van der Molen, Jan C. ;

Links, Thera P. ;

Morris, Michael R. ;

Ross, H. Alec ;

de Vries, Elisabeth G. E. ;

Kema, Ido P. .

CLINICAL CHEMISTRY, 2007, 53 (09) :1684-1693

← 1 2 3 4 5 →