Effects of chronic isovolaemic haemodilution on regional cerebral blood flow in conscious rats

被引:3
作者
Frietsch, T
Lenz, C
Kuschinsky, W
Waschke, KE
机构
[1] Univ Heidelberg, Fac Clin Med Mannheim, Dept Anesthesiol & Crit Care Med, D-68167 Mannheim, Germany
[2] Univ Heidelberg, Dept Physiol & Pathophysiol, D-68167 Mannheim, Germany
关键词
clinical laboratory techniques; cytological techniques; autoradiography; haemodilution; haemodynamics; regional blood flow; brain; rats; Sprague-Dawley;
D O I
10.1017/S0265021504001097
中图分类号
R614 [麻醉学];
学科分类号
100217 ;
摘要
Background and objective: Acute isovolaemic haemodilution increases local and mean cerebral blood flow. It is not known whether a single haemodilution has a short-term effect only or whether it affects cerebral perfusion over a longer time period. In the present study, local and mean cerebral blood flow were determined in conscious rats after a 4, 24 and 48 h period following one-time haemodilution. Methods: Thirty-six rats were randomized to three untreated sham groups and three groups of haemodilution (4, 24 or 48 h, n = 6 for each group). Isovolaemic haemodilution with albumin 5% aimed to a target haematocrit of 0.2. Local cerebral blood flow was measured in 38 brain regions by the iodo-[C-14]antipyrine method in conscious normothermic rats. Results: Isovolaemic haemodilution reduced haematocrit from 0.44 to 0.20. During the following 24 and 48 h periods, haematocrit remained low (0.22 and 0.21). Mean cerebral blood flow was similar in untreated sham groups (88 +/- 12 after 4 h, 92 +/- 11 after 24 h, 96 +/- 10 mL 100 g(-1) min(-1) after 48 h). Haemodilution increased mean cerebral blood flow after 4 h (184 +/- 11 mL 100 g(-1) min(-1)), after 24 h (153 +/- 13 mL 100 g(-1) min(-1)) and 48 h (149 +/- 15 mL 100 g(-1) min(-1)) (P less than or equal to 0.05). Local cerebral blood flow increased in all 38 structures after 4 h haemodilution but decreased with time in six of 38 brain structures after 24 h and in 15 regions after 48h (P less than or equal to 0.05). Conclusions: A single one-time haemodilution increased mean cerebral blood flow for 2 days. However, local adaptation of cerebral blood flow to a chronic low haematocrit occurred but was heterogeneous within the brain.
引用
收藏
页码:53 / 59
页数:7
相关论文
共 18 条
[1]  
ASPLUND K, 1987, STROKE, V18, P691
[2]   Effect of delayed albumin hemodilution on infarction volume and brain edema after transient middle cerebral artery occlusion in rats [J].
Belayev, L ;
Busto, R ;
Zhao, WZ ;
Clemens, JA ;
Ginsberg, MD .
JOURNAL OF NEUROSURGERY, 1997, 87 (04) :595-601
[3]   Effects of luminal shear stress on cerebral arteries and arterioles [J].
Bryan, RM ;
Marrelli, SP ;
Steenberg, ML ;
Schildmeyer, LA ;
Johnson, TD .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2001, 280 (05) :H2011-H2022
[4]   EFFECTS OF VISCOSITY AND OXYGEN-CONTENT ON CEREBRAL BLOOD-FLOW IN ISCHEMIC AND NORMAL RAT-BRAIN [J].
COLE, DJ ;
DRUMMOND, JC ;
PATEL, PM ;
MARCANTONIO, S .
JOURNAL OF THE NEUROLOGICAL SCIENCES, 1994, 124 (01) :15-20
[5]  
GOSLINGA H, 1991, ACTA MED AUSTRIACA, V1, P41
[6]   Morphologic responses of endothelium to shear stress: Reorganization of the adherens junction [J].
Langille, BL .
MICROCIRCULATION, 2001, 8 (03) :195-206
[7]   Transient and steady-state effects of shear stress on endothelial cell adherens junctions [J].
Noria, S ;
Cowan, DB ;
Gotlieb, AI ;
Langille, BL .
CIRCULATION RESEARCH, 1999, 85 (06) :504-514
[8]   Effects of preoperative intentional hemodilution on the extravasation rate of albumin and fluid [J].
Payen, JF ;
Vuillez, JP ;
Geoffray, B ;
Lafond, JL ;
Comet, M ;
Stieglitz, P ;
Jacquot, C .
CRITICAL CARE MEDICINE, 1997, 25 (02) :243-248
[9]   ALBUMIN EXTRAVASATION RATES IN TISSUES OF ANESTHETIZED AND UNANESTHETIZED RATS [J].
RENKIN, EM ;
JOYNER, WL ;
GUSTAFSONSGRO, M ;
PLOPPER, G ;
SIBLEY, L .
JOURNAL OF APPLIED PHYSIOLOGY, 1989, 66 (05) :2056-2060
[10]   Mechanotransduction in endothelial cells: Temporal signaling events in response to shear stress [J].
Takahashi, M ;
Ishida, T ;
Traub, O ;
Corson, MA ;
Berk, BC .
JOURNAL OF VASCULAR RESEARCH, 1997, 34 (03) :212-219