Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer

被引：179

作者：

Brisson, Lucie ^{[1
]}

Banski, Piotr ^{[1
]}

Sboarina, Martina ^{[1
]}

Dethier, Coralie ^{[1
]}

Danhier, Pierre ^{[1
,2
]}

Fontenille, Marie-Josephine ^{[1
]}

Van Hee, Vincent F. ^{[1
]}

Vazeille, Thibaut ^{[1
]}

Tardy, Morgane ^{[1
]}

Falces, Jorge ^{[1
]}

Bouzin, Caroline ^{[3
]}

Porporato, Paolo E. ^{[1
]}

Frederick, Raphael ^{[2
]}

Michiels, Carine ^{[4
]}

Copetti, Tamara ^{[1
]}

Sonveaux, Pierre ^{[1
]}

机构：

[1] Catholic Univ Louvain, Pole Pharmacol, Inst Rech Expt & Clin, B-1200 Brussels, Belgium

[2] Catholic Univ Louvain, Louvain Drug Res Inst, B-1200 Brussels, Belgium

[3] Catholic Univ Louvain, IREC Imaging Platform, B-1200 Brussels, Belgium

[4] Univ Namur, URBC NARILIS, B-5000 Namur, Belgium

来源：

CANCER CELL | 2016年 / 30卷 / 03期

基金：

澳大利亚研究理事会;

关键词：

BREAST-CANCER; CELL-DEATH; TUMOR-CELLS; H SUBUNIT; EXPRESSION; PROTEIN; MITOCHONDRIAL; CHLOROQUINE; GROWTH; ANGIOGENESIS;

D O I：

10.1016/j.ccell.2016.08.005

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Metabolic adaptability is essential for tumor progression and includes cooperation between cancer cells with different metabolic phenotypes. Optimal glucose supply to glycolytic cancer cells occurs when oxidative cancer cells use lactate preferentially to glucose. However, using lactate instead of glucose mimics glucose deprivation, and glucose starvation induces autophagy. We report that lactate sustains autophagy in cancer. In cancer cells preferentially to normal cells, lactate dehydrogenase B (LDHB), catalyzing the conversion of lactate and NAD(+) to pyruvate, NADH and H+, controls lysosomal acidification, vesicle maturation, and intracellular proteolysis. LDHB activity is necessary for basal autophagy and cancer cell proliferation not only in oxidative cancer cells but also in glycolytic cancer cells.

引用

页码：418 / 431

页数：14

共 67 条

[11] Macropinocytosis of protein is an amino acid supply route in Ras-transformed cells [J].

Commisso, Cosimo ;

Davidson, Shawn M. ;

Soydaner-Azeloglu, Rengin G. ;

Parker, Seth J. ;

Kamphorst, Jurre J. ;

Hackett, Sean ;

Grabocka, Elda ;

Nofal, Michel ;

Drebin, Jeffrey A. ;

Thompson, Craig B. ;

Rabinowitz, Joshua D. ;

Metallo, Christian M. ;

Vander Heiden, Matthew G. ;

Bar-Sagi, Dafna .

NATURE, 2013, 497 (7451) :633-+

[12] Cancer metabolism, stemness and tumor recurrence: MCT1 and MCT4 are functional biomarkers of metabolic symbiosis in head and neck cancer [J].

Curry, Joseph M. ;

Tuluc, Madalina ;

Whitaker-Menezes, Diana ;

Ames, Julie A. ;

Anantharaman, Archana ;

Butera, Aileen ;

Leiby, Benjamin ;

Cognetti, David M. ;

Sotgia, Federica ;

Lisanti, Michael P. ;

Martinez-Outschoorn, Ubaldo E. .

CELL CYCLE, 2013, 12 (09) :1371-1384

[13] Molecular risk stratification of medulloblastoma patients based on immunohistochemical analysis of MYC, LDHB, and CCNB1 expression [J].

de Haas, Talitha ;

Hasselt, Nancy ;

Troost, Dirk ;

Caron, Huib ;

Popovic, Mara ;

Zadravec-Zaletel, Lorna ;

Grajkowska, Wieslawa ;

Perek, Marta ;

Osterheld, Maria-Chiara ;

Ellison, David ;

Baas, Frank ;

Versteeg, Rogier ;

Kool, Marcel .

CLINICAL CANCER RESEARCH, 2008, 14 (13) :4154-4160

[14] Lactate Activates HIF-1 in Oxidative but Not in Warburg-Phenotype Human Tumor Cells [J].

De Saedeleer, Christophe J. ;

Copetti, Tamara ;

Porporato, Paolo E. ;

Verrax, Julien ;

Feron, Olivier ;

Sonveaux, Pierre .

PLOS ONE, 2012, 7 (10)

[15] Lactate Dehydrogenase B: A Metabolic Marker of Response to Neoadjuvant Chemotherapy in Breast Cancer [J].

Dennison, Jennifer B. ;

Molina, Jennifer R. ;

Mitra, Shreya ;

Gonzalez-Angulo, Ana M. ;

Balko, Justin M. ;

Kuba, Maria G. ;

Sanders, Melinda E. ;

Pinto, Joseph A. ;

Gomez, Henry L. ;

Arteaga, Carlos L. ;

Brown, Robert E. ;

Mills, Gordon B. .

CLINICAL CANCER RESEARCH, 2013, 19 (13) :3703-3713

[16] Cycling hypoxia and free radicals regulate angiogenesis and radiotherapy response [J].

Dewhirst, Mark W. ;

Cao, Yiting ;

Moeller, Benjamin .

NATURE REVIEWS CANCER, 2008, 8 (06) :425-437

[17] Multiple Biological Activities of Lactic Acid in Cancer: Influences on Tumor Growth, Angiogenesis and Metastasis [J].

Dhup, Suveera ;

Dadhich, Rajesh Kumar ;

Porporato, Paolo Ettore ;

Sonveaux, Pierre .

CURRENT PHARMACEUTICAL DESIGN, 2012, 18 (10) :1319-1330

[18] Endothelial nitric oxide synthase targeting to caveolae - Specific interactions with caveolin isoforms in cardiac myocytes and endothelial cells [J].

Feron, O ;

Belhassen, L ;

Kobzik, L ;

Smith, TW ;

Kelly, RA ;

Michel, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (37) :22810-22814

[19] The Atg8 and Atg12 ubiquitin-like conjugation systems in macroautophagy [J].

Geng, Jiefei ;

Klionsky, Daniel J. .

EMBO REPORTS, 2008, 9 (09) :859-864

[20] Strengthened glycolysis under hypoxia supports tumor symbiosis and hexosamine biosynthesis in pancreatic adenocarcinoma [J].

Guillaumond, Fabienne ;

Leca, Julie ;

Olivares, Orianne ;

Lavaut, Marie-Noelle ;

Vidal, Nicolas ;

Berthezene, Patrice ;

Dusetti, Nelson Javier ;

Loncle, Celine ;

Calvo, Ezequiel ;

Turrini, Olivier ;

Iovanna, Juan Lucio ;

Tomasini, Richard ;

Vasseur, Sophie .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (10) :3919-3924

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