A radiation hybrid map of river buffalo (Bubalus bubalis) chromosome 7 and comparative mapping to the cattle and human genomes

被引：15
作者：
Goldammer, T. [1 ]
Weikard, R. [1 ]
Miziara, M. N. [2 ]
Brunner, R. M. [1 ]
Agarwala, R. [3 ]
Schaeffer, A. A. [3 ]
Womack, E. [4 ]
Amaral, M. E. J. [2 ,4 ]
机构：
[1] Forsch Inst Biol Landwirtschaftlicher Nutztiere, Forschungsereich Mol Biol, D-18196 Dummerstorf, Germany
[2] UNESP, IBILCE, Inst Biociencias, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil
[3] Natl Ctr Biotechnol Informat, NIH, Dept Hlth & Human Services, Bethesda, MD USA
[4] Texas A&M Univ, Dept Vet Pathobiol, College Stn, TX USA
来源：
CYTOGENETIC AND GENOME RESEARCH | 2007年 / 119卷 / 3-4期
关键词：
D O I：
10.1159/000112067
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A preliminary radiation hybrid (RH) map containing 50 loci on chromosome 7 of the domestic river buffalo Bubalus bubalis (BBU; 2n = 50) was constructed based on a comparative mapping approach. The RH map of BBU7 includes thirty-seven gene markers and thirteen microsatellites. All loci have been previously assigned to Bos taurus (BTA) chromosome BTA6, which is known for its association with several economically important milk production traits in cattle. The map consists of two linkage groups spanning a total length of 627.9 cR(5,000). Comparative analysis of the BBU7 RH 5,000 map with BTA6 in cattle gave new evidence for strong similarity between the two chromosomes over their entire length and exposed minor differences in locus order. Comparison of the BBU7 RH 5,000 map with the Homo sapiens (HSA) genome revealed similarity with a large chromosome segment of HSA4. Comparative analysis of loci in both species revealed more variability than previously known in gene order and several chromosome rearrangements including centromere relocation. The data obtained in our study define the evolutionarily conserved segment on BBU7 and HSA4 to be between 3.5 megabases (Mb) and 115.8 Mb in the HSA4 (genome build 36) DNA sequence. Copyright (c) 2008 S. Karger AG, Basel.
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页码：235 / 241
页数：7
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