Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis

被引：26
作者：
Chen, Tingting [1 ]
Wang, Yong [2 ]
Yang, Yang [1 ]
Yu, Kaikai [3 ]
Cao, Xiangliao [1 ]
Su, Fang [1 ]
Xu, Huanbai [4 ]
Peng, Yongde [4 ]
Hu, Yudong [3 ]
Qian, Feng [1 ,3 ]
Wang, Zishu [1 ]
机构：
[1] Bengbu Med Coll, Anhui Prov Key Lab Translat Canc Res, Affiliated Hosp 1, Dept Med Oncol, 287 Changhuai Rd, Bengbu 233004, Anhui, Peoples R China
[2] Shanghai Hlth Med Coll, Zhoupu Hosp, Dept Gen Surg, Shanghai 201318, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Pharm, Minist Educ, Engn Res Ctr Cell & Therapeut Antibody, 800 Dongchuan Rd, Shanghai 200240, Peoples R China
[4] Shanghai Jiao Tong Univ, Sch Med, Shanghai Gen Hosp, Dept Endocrinol & Metab, Shanghai 200080, Peoples R China
来源：
BIOLOGICAL RESEARCH | 2019年 / 52卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Gramicidin; Proliferation; Apoptosis; Cell cycle arrest; SGC-7901; N-FORMYLATED GRAMICIDIN; MITOCHONDRIA; GROWTH; FOXO1;
D O I：
10.1186/s40659-019-0264-1
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Background: Gastric cancer is a common malignant tumor with high morbidity and mortality worldwide, which seriously affects human health. Gramicidin is a short peptide antibiotic which could be used for treating infection induced by bacteria or fungi. However, the anti-cancer effect of gramicidin on gastric cancer cells and its underlying mechanism remains largely unknown. Results: Gastric cancer cells SGC-7901, BGC-823 and normal gastric mucosal cells GES-1 were treated with different concentrations of gramicidin respectively. The results of CCK-8 experiment revealed cellular toxicity of gramicidin to cancer cells while cell colony formation assay showed that gramicidin significantly inhibited the proliferation of gastric cancer cells, but had little effect on normal gastric mucosal cells. In addition, the wound healing assay showed that gramicidin inhibited the migration of SGC-7901 cell. Meanwhile, apoptosis and cell cycle analysis revealed that gramicidin induced cell apoptosis with G2/M cell cycle inhibition. Furthermore, western blot analysis demonstrated that gramicidin down-regulated the expression of cyclinD1 and Bcl-2 as well as the FoxO1 phosphorylation. Conclusions: The current study illustrated the anti-tumor activity of gramicidin on gastric cancer cells, providing a possibility for gramicidin to be applied in clinical practice for the treatment of gastric cancer.
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页数：11
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