Purification and characterization of an exo-polygalacturonase produced by Penicillium viridicatum RFC3 in solid-state fermentation

被引：33

作者：

Silva, Denis ^{[1
]}

Martins, Eduardo Silva ^{[1
]}

Leite, Rodrigo Simoes Ribeiro ^{[1
]}

Da Silva, Roberto ^{[1
]}

Ferreira, Viviani ^{[1
]}

Gomes, Eleni ^{[1
]}

机构：

[1] Sao Paulo State Univ Unesp, IBILCE, Dept Biol, Lab Biochem & Appl Microbiol, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil

来源：

PROCESS BIOCHEMISTRY | 2007年 / 42卷 / 08期

基金：

巴西圣保罗研究基金会;

关键词：

polygalacturonase; penicillum; solid-state fermentation; purification;

D O I：

10.1016/j.procbio.2007.05.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pectinolytic enzyme obtained from Penicillium viridicatum RFC by solid-state fermentation was purified to homogeneity by pretreatment with kaolin (40 mg mL(-1) ) and ultrafiltration. followed by chromatography on a Sephadex G50 column. The apparent molecular weight of the enzyme was 24 kDa. Maximal activity occurred at pH 6.0 and at 60 degrees C. The enzyme proved to be an exo-polygalacturonase, releasing galacturonic acid by hydrolysis of highly esterified pectin. The presence of 10 mM Ba2+ increased the enzyme activity by 96% and its thermal stability by 30%. besides increasing its stability at acid pH. The apparent K-m with apple pectin as substrate was 1.82 mg mL(-1) and the V-max was 81 mu mol min(-1). (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1237 / 1243

页数：7

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