G protein gamma 7 suppresses progression of lung adenocarcinoma by inhibiting E2F transcription factor 1

被引：13

作者：

Zheng, Hongyu ^{[1
]}

Tian, Hui ^{[1
]}

Yu, Xuejuan ^{[1
]}

Ren, Peng ^{[1
]}

Yang, Qiuan ^{[1
]}

机构：

[1] ShanDong Univ, Dept Radiat Oncol, QiLu Hosp, 107 Wenhua West Rd, Jinan 250012, Shandong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2021年 / 182卷

关键词：

Lung adenocarcinoma; GNG7; CANCER; IDENTIFICATION; EXPRESSION; ROLES;

D O I：

10.1016/j.ijbiomac.2021.04.082

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

G protein gamma 7 (GNG7) has been found to be aberrantly expressed in some kinds of malignant tumors. In this study, we mainly discuss the antitumor role of it in lung adenocarcinoma (LUAD) cells. Protein levels of GNG7 in LUAD tissues were measured by western blot and immunohistochemical analysis. Cell proliferation, invasion and migration were detected by CCK-8 assay, 5-ethynyl-2 '-deoxyuridine (EdU), and Transwell assay. In our study, GNG7 was down-regulated in LUAD, which significantly correlated with survival of LUAD patients. Functional experiments revealed that GNG7 significantly inhibited LUAD cell proliferation, migration, and invasion in vitro and E2F1 overexpression reversed these properties. GNG7 suppressed xenograft tumorigenesis in nude mice models in vivo. In conclusion, GNG7 functions as a tumor suppressor in LUAD cells through inhibiting E2F1. (c) 2021 Published by Elsevier B.V.

引用

页码：858 / 865

页数：8

共 28 条

[1] Emerging roles of E2Fs in cancer: an exit from cell cycle control [J].

Chen, Hui-Zi ;

Tsai, Shih-Yin ;

Leone, Gustavo .

NATURE REVIEWS CANCER, 2009, 9 (11) :785-797

[2] Early2 factor (E2F) deregulation is a prognostic and predictive biomarker in lung adenocarcinoma [J].

Chen, Lu ;

Kurtyka, Courtney A. ;

Welsh, Eric A. ;

Rivera, Jason I. ;

Engel, Brienne E. ;

Munoz-Antonia, Teresita ;

Yoder, Sean J. ;

Eschrich, Steven A. ;

Creelan, Ben C. ;

Chiappori, Alberto A. ;

Gray, Jhanelle E. ;

Luis Ramirez, Jose ;

Rosell, Rafael ;

Schabath, Matthew B. ;

Haura, Eric B. ;

Chen, Dung-Tsa ;

Cress, W. Douglas .

ONCOTARGET, 2016, 7 (50) :82254-82265

[3] Long Noncoding RNA SBF2-AS1 Is Critical for Tumorigenesis of Early-Stage Lung Adenocarcinoma [J].

Chen, Rui ;

Xia, Wenjia ;

Wang, Siwei ;

Xu, Youtao ;

Ma, Zhifei ;

Xu, Weizhang ;

Zhang, Erbao ;

Wang, Jie ;

Fang, Tian ;

Zhang, Quan'an ;

Dong, Gaochao ;

Cho, William Chi-shing ;

Ma, Patrick C. ;

Brandi, Giovanni ;

Tavolari, Simona ;

Ujhazy, Peter ;

Metro, Giulio ;

Popper, Helmut H. ;

Yin, Rong ;

Qiu, Mantang ;

Xu, Lin .

MOLECULAR THERAPY NUCLEIC ACIDS, 2019, 16 :543-553

[4] Identification of guanine nucleotide-binding protein γ-7 as an epigenetically silenced gene in head and neck cancer by gene expression profiling [J].

Demokan, Semra ;

Chuang, Alice Y. ;

Chang, Xiaofei ;

Khan, Tanbir ;

Smith, Ian M. ;

Pattani, Kavita M. ;

Dasgupta, Santanu ;

Begum, Shahnaz ;

Khan, Zubair ;

Liegeois, Nanette J. ;

Westra, William H. ;

Sidransky, David ;

Koch, Wayne ;

Califano, Joseph A. .

INTERNATIONAL JOURNAL OF ONCOLOGY, 2013, 42 (04) :1427-1436

[5] miR-218 suppresses gastric cancer cell cycle progression through the CDK6/Cyclin D1/E2F1 axis in a feedback loop [J].

Deng, Min ;

Zeng, Chao ;

Lu, Xihong ;

He, Xiusheng ;

Zhang, Ruixin ;

Qiu, Qinwei ;

Zheng, Guopei ;

Jia, Xiaoting ;

Liu, Hao ;

He, Zhimin .

CANCER LETTERS, 2017, 403 :175-185

[6] Comprehensive bioinformatics analysis identifies several potential diagnostic markers and potential roles of cyclin family members in lung adenocarcinoma [J].

Gao, Li-Wei ;

Wang, Guo-Liang .

ONCOTARGETS AND THERAPY, 2018, 11 :7407-7415

[7] KLF6 Suppresses Metastasis of Clear Cell Renal Cell Carcinoma via Transcriptional Repression of E2F1 [J].

Gao, Yu ;

Li, Hongzhao ;

Ma, Xin ;

Fan, Yang ;

Ni, Dong ;

Zhang, Yu ;

Huang, Qingbo ;

Liu, Kan ;

Li, Xintao ;

Wang, Lei ;

Gu, Liangyou ;

Yao, Yuanxin ;

Ai, Qing ;

Du, Qingshan ;

Song, Erlin ;

Zhang, Xu .

CANCER RESEARCH, 2017, 77 (02) :330-342

[8] RFC3 induces epithelial-mesenchymal transition in lung adenocarcinoma cells through the Wnt/β-catenin pathway and possesses prognostic value in lung adenocarcinoma [J].

Gong, Shulei ;

Qu, Xiaohan ;

Yang, Shize ;

Zhou, Siyu ;

Li, Peiwen ;

Zhang, Qigang .

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2019, 44 (06) :2276-2288

[9]

Guo X., 2019, EXP CELL RES, V382, P10

[10] Early Lung Cancer Detection [J].

Inage, Terunaga ;

Nakajima, Takahiro ;

Yoshino, Ichiro ;

Yasufuku, Kazuhiro .

CLINICS IN CHEST MEDICINE, 2018, 39 (01) :45-+

← 1 2 3 →