Interaction of pea (Pisum sativum L.) lectins with rhizobial strains

被引：7
|
作者：
Bajaj, M [1 ]
Soni, G [1 ]
Singh, CK [1 ]
机构：
[1] Punjab Agr Univ, Dept Biochem, Ludhiana 141004, Punjab, India
来源：
MICROBIOLOGICAL RESEARCH | 2001年 / 156卷 / 01期
关键词：
lectins; pea (Pisum sativum L.); rhizobium;
D O I：
10.1078/0944-5013-00080
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Lectins from two varieties (PG-3 and LFP-48) of pea have been purified by affinity chromatography on Sephadex G-50. The specific activity increased by 23 and 25 folds, respectively. These lectins from both the varieties were found to be specific for mannose. The purified fluorescein isothiocyanate (FITC)- labelled lectins showed binding reaction with homologous as well as heterologous strains of Rhizobium spp. The results revealed that pea lectins are not highly specific to their respective rhizobia. Moreover, these lectins showed a greater stimulatory effect on homologous Rhizobium leguminosarum strains.
引用
收藏
页码：71 / 74
页数：4
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