Gomisin N from Schisandra chinensis Ameliorates Lipid Accumulation and Induces a Brown Fat-Like Phenotype through AMP-Activated Protein Kinase in 3T3-L1 Adipocytes

被引:17
|
作者
Lee, Kippeum [1 ]
Lee, Yeon-Joo [1 ]
Kim, Kui-Jin [1 ]
Chei, Sungwoo [1 ]
Jin, Heegu [1 ]
Oh, Hyun-Ji [1 ]
Lee, Boo-Yong [1 ]
机构
[1] CHA Univ, Coll Life Sci, Dept Food Sci & Biotechnol, Seongnam 13488, Kyonggi Do, South Korea
关键词
gomisin N; 3T3-L1; lipid accumulation; browning; obesity; ADIPOSE-TISSUE; ENERGY-METABOLISM; WHITE; ALPHA; LIPOPOLYSACCHARIDE; DYSFUNCTION; EXPRESSION; LIGNANS; PRDM16; CELLS;
D O I
10.3390/ijms21062153
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Obesity results from an imbalance between energy intake and energy expenditure, in which excess fat is stored as triglycerides (TGs) in white adipocytes. Recent studies have explored the anti-obesity effects of certain edible phytochemicals, which suppress TG accumulation and stimulate a brown adipocyte-like phenotype in white adipocytes. Gomisin N (GN) is an important bioactive component of Schisandra chinensis, a woody plant endemic to Asia. GN has antioxidant, anti-inflammatory and hepatoprotective effects in vivo and in vitro. However, the anti-obesity effects of GN in lipid metabolism and adipocyte browning have not yet been investigated. In the present study, we aimed to determine whether GN suppresses lipid accumulation and regulates energy metabolism, potentially via AMP-activated protein kinase (AMPK), in 3T3-L1 adipocytes. Our findings demonstrate that GN inhibited adipogenesis and lipogenesis in adipocyte differentiation. Also, GN not only increased the expression of thermogenic factors, including uncoupling protein 1 (UCP1), but also enhanced fatty acid oxidation (FAO) in 3T3-L1 cells. Therefore, GN may have a therapeutic benefit as a promising natural agent to combat obesity.
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页数:14
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