No significant expression of CYP2E1 in rat liver stellate cells

被引：12
|
作者：
Oinonen, T
Koivisto, T
Lindros, KO
机构：
[1] Natl Publ Hlth Inst, Alcohol Res Ctr, Helsinki 00101, Finland
[2] Univ Helsinki, Cent Hosp, Alcohol Dis Res Unit, Helsinki, Finland
来源：
BIOCHEMICAL PHARMACOLOGY | 1998年 / 56卷 / 08期
关键词：
CYP2E1; expression; rat liver; stellate cell isolation; collagen; 1; fibrogenesis;
D O I：
10.1016/S0006-2952(98)00062-8
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The putative role of the ethanol-inducible cytochrome P450(CYP)2E1 in stimulating collagen synthesis by rat liver stellate cells was studied. Analysis of carefully isolated stellate cells revealed that their content of immunoreactive CYP2E1 protein and of CYP2E1 mRNA, as determined by reverse transcription-polymerase chain reaction (RT-PCR), was very low, i.e. only 0-4% of that in hepatocytes. We conclude that it is improbable that such low expression of CYP2E1 in stellate cells would have functional importance. (C) 1998 Elsevier Science Inc.
引用
收藏
页码：1075 / 1078
页数：4
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