Development and validation of a GC-MS method for rapid determination of galanthamine in Leucojum aestivum and Narcissus ssp.: A metabolomic approach

被引:59
作者
Berkov, Strahil [1 ,2 ]
Bastida, Jaume [1 ]
Viladomat, Francesc [1 ]
Codina, Carles [1 ]
机构
[1] Univ Barcelona, Dept Prod Nat Biol Vegetal & Edafol, Fac Farm, E-08028 Barcelona, Spain
[2] AgroBioInst, Sofia 1164, Bulgaria
关键词
Galanthamine; Narcissus; Leucojum; In vitro; Metabolomics; Method validation; GC-MS; CHROMATOGRAPHY-MASS-SPECTROMETRY; QUANTITATIVE-DETERMINATION; AMARYLLIDACEAE ALKALOIDS; LIQUID-CHROMATOGRAPHY; GALANTAMINE; METABOLITES; DIFFERENTIATION; PLANTS;
D O I
10.1016/j.talanta.2010.11.029
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Galanthamine, an acetylcholinesterase inhibitor marketed as a hydrobromide salt for the treatment of Alzheimer's disease, is obtained from some Amaryllidaceae plants. A new method was developed and validated for its quantification by GC-MS in different plant sources: bulbs and leaves from Narcissus confusus; bulbs from N. pseudonarcissus cv. Carlton; and leaves and in vitro cultures from L aestivum. Samples (50 mg) were extracted with methanol (1 mL) for 2 h, then aliquots of the extracts were silylated and analyzed by GC-MS. The calibration line was linear over a range of 15-800 mu g galanthamine/sample, ensuring an analysis of samples with a content of 0.03-1.54% analyte referred to dry weight. The recovery was generally more than 95%. Good inter- and intra assay precision was observed (RSD < 3%). Principal component analysis of GC-MS chromatograms allowed discrimination of the plant raw material with respect to species, organs and geographical regions. The analytical method developed in this study proved to be simple, sensitive and far more informative than the routine analytical methods (GC, HPLC, CE and NMR), so it may be useful for quality control of plant raw materials in the pharmaceutical industry. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:1455 / 1465
页数:11
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