Quantitative Estimation of IL-6 in Serum/Plasma Samples Using a Rapid and Cost-Effective Fiber-Optic dip-probe

被引：1

作者：

Wang, Chun Wei ^{[1
]}

Manne, Upender ^{[2
]}

Reddy, Vishnu B. ^{[2
]}

Kapoor, Rakesh ^{[1
]}

机构：

[1] Univ Alabama Birmingham, Dept Phys, Birmingham, AL 35294 USA

[2] Univ Alabama Birmingham, Dept Phys & Pathol, Birmingham, AL 35294 USA

来源：

OPTICAL FIBERS AND SENSORS FOR MEDICAL DIAGNOSTICS AND TREATMENT APPLICATIONS X | 2010年 / 7559卷

关键词：

Fiber-optic; Fluorescence; IL-6; Cytokine; Serum; lupus; lymphoma; BIOSENSOR;

D O I：

10.1117/12.843807

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A rapid and cost-effective combination tapered fiber-optic biosensor (CTFOB) dip-probe was used for quantitative estimation of interleukin (IL)-6 in serum/plasma samples. Sandwich immunoassay was used as the detection technique. Probes could successfully detect presence of IL-6 in two serum samples, non-neoplastic autoimmune patient (lupus) sample and lymphoma patient sample. The estimated amount of IL-6 in lupus patient sample was 4.8 +/- 0.9 pM and in lymphoma patient sample was 2 +/- 1 pM. It is demonstrated that the developed CTFOB dip-probe is capable of quantitative estimation of proteins in serum/plasma samples with high specificity.

引用

页数：5

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