Isolation and characterization of a polygalacturonase-inhibiting protein from potato leaves. Accumulation in response to salicylic acid, wounding and infection

被引：30

作者：

Machinandiarena, MF ^{[1
]}

Olivieri, FP ^{[1
]}

Daleo, GR ^{[1
]}

Oliva, CR ^{[1
]}

机构：

[1] Univ Mar del Plata, Fac Ciencias Exactas & Nat, Inst Invest Biol, RA-7600 Mar Del Plata, Argentina

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2001年 / 39卷 / 02期

关键词：

Phytophthora infestans; plant defence; polygalacturonase-inhibiting protein; Solanum tuberosum;

D O I：

10.1016/S0981-9428(00)01228-6

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A polygalacturonase-inhibiting protein (PGIP) was purified from potato leaves (Solanum tuberosum L. cv. Spunta). The protein was cell wall bound and had a molecular ma ss of 41 kDa, as determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and gel filtration. Potato PGIP showed a broad inhibitory activity against crude polygalacturonase preparations from several fungi grown in liquid culture. Using heterologous antibodies in western blot experiments, it was shown that the levels of PGIP are increased in potato leaves in response to wounding, treatment with salicylic acid and infection with Phytophthora infestans during an incompatible interaction. The data support the hypothesis that, synthesis of PGIP may constitute an active defence mechanism of potato plants that is elicited by signal molecules known to induce plant defence genes. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

引用

页码：129 / 136

页数：8

共 40 条

[1] THE INVIVO ROLE OF BARTLETT PEAR FRUIT POLYGALACTURONASE INHIBITORS [J].