Genetic variation of sweet potatoes (Ipomoea batatas L) cultivated in Chile determined by RAPDs

被引：16

作者：

Sagredo, B ^{[1
]}

Hinrichsen, P ^{[1
]}

López, H ^{[1
]}

Cubillos, A ^{[1
]}

Muñoz, C ^{[1
]}

机构：

[1] Inst Invest Agropecuarias, Estac Expt La Platina, Santiago, Chile

来源：

EUPHYTICA | 1998年 / 101卷 / 02期

关键词：

sweet potato; Ipomoea batatas; RAPD; genetic variation; Chile;

D O I：

10.1023/A:1018301009296

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

RAPD (randomly amplified polymorphic DNA) technology was applied to analyze the genetic variability of sweet potato germplasm existing in Chile and elsewhere. Analysis of 28 cultivars from all over the world showed polymorphic bands with all 18 primers tested. A total of 124 RAPD bands were scored with an average of 6.9 polymorphic bands per primer. These results confirm that sweet potato exhibits high genetic variation. Two groups were distinguished: one containing Peruvian cultivars, and another containing cultivars from the rest of the world. Analysis of 14 accessions from Central Chile and one from Northern Chile showed polymorphic bands with 24 of 26 primers tested, but almost all of the 140 polymorphic bands merely showed the distinctness of the Northern accession. The almost complete uniformity of the other 14 accessions shows that sweet potato germplasm collected in Central Chile has very little genetic variability and may be derived from a single cultivar. Based on these results and on historical records, some hypotheses are proposed to explain the origin of sweet potatoes cultivated in Chile.

引用

页码：193 / 198

页数：6

共 27 条

[11] Gene pool classification of common bean landraces from Chile based on RAPD and morphological data
Johns, MA
Skroch, PW
Nienhuis, J
Hinrichsen, P
Bascur, G
MunozSchick, C
[J]. CROP SCIENCE, 1997, 37 (02) : 605 - 613
[12] LIZARRAGA R, 1990, 32 CIP
[13] LOVE JE, 1978, HORTSCIENCE, V13, P578
[14] INFRASPECIFIC DIFFERENTIATION OF GARLIC (ALLIUM-SATIVUM L) BY ISOZYME AND RAPD MARKERS
MAASS, HI
KLAAS, M
[J]. THEORETICAL AND APPLIED GENETICS, 1995, 91 (01) : 89 - 97
[15] MONTALDO P, 1988, AGROSUR, V16, P132
[16] Nishiyama I., 1982, Sweet potato. Proceedings of the first international symposium, Taiwan, 23-27 March 1981., P263
[17] Nunez L., 1989, CULTURAS CHILE PREHI, P81
[18] The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis
Powell, W
Morgante, M
Andre, C
Hanafey, M
Vogel, J
Tingey, S
Rafalski, A
[J]. MOLECULAR BREEDING, 1996, 2 (03) : 225 - 238
[19] ROHLF FJ, 1993, MONOGRAPHY
[20] Sambrook J., 1989, MOL CLONING

← 1 2 3 →