Varicella-zoster virus gene 66 transcription and translation din latently infected human ganglia

被引:91
作者
Cohrs, RJ
Gilden, DH
Kinchington, PR
Grinfeld, E
Kennedy, PGE
机构
[1] Univ Colorado, Hlth Sci Ctr, Dept Neurol, Denver, CO 80262 USA
[2] Univ Colorado, Hlth Sci Ctr, Dept Microbiol, Denver, CO 80262 USA
[3] Univ Pittsburgh, Dept Ophthalmol, Pittsburgh, PA 15213 USA
[4] Univ Glasgow, Dept Neurol, Glasgow G51 4TF, Lanark, Scotland
关键词
D O I
10.1128/JVI.77.12.6660-6665.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Latent infection with varicella-zoster virus (VZV) is characterized by restricted virus gene expression and the absence of virus production. Of the similar to70 predicted VZV genes, only five (genes 4, 21, 29, 62, and 63) have been shown by multiple techniques to be transcribed during latency. IE62, the protein product of VZV gene 62, is the major immediate-early (IE) virus-encoded transactivator of viral gene transcription and plays a pivotal role in transactivating viral genes during lytic infection. The protein kinase (66-pk) encoded by VZV gene 66 phosphorylates IE62, resulting in cytoplasmic accumulation of IE62 that mitigates nuclear IE62-induced gene activation. Analysis of latently infected human trigeminal ganglia for 66-pk expression by reverse transcriptase-dependent nested PCR, including DNA sequence analysis, in situ hybridization, and immunohistochemistry, revealed VZV open reading frame 66 to be a previously unrecognized latently expressed virus gene and suggests that prevention of IE62 import to the nucleus by VZV 66-pk phosphorylation is one possible mechanism by which VZV latency is maintained.
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收藏
页码:6660 / 6665
页数:6
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