Development of a complete set of disomic rape-radish chromosome-addition lines

被引：45

作者：

Budahn, H. ^{[1
]}

Schrader, O. ^{[1
]}

Peterka, H. ^{[1
]}

机构：

[1] Fed Ctr Breeding Res Cultivated Plants, Inst Hort Crops, D-06484 Quedlinburg, Germany

来源：

EUPHYTICA | 2008年 / 162卷 / 01期

关键词：

Brassica napus; Raphanus sativus; FISH; chromosome-specific marker; interspecific hybridization; monosomic alien addition line;

D O I：

10.1007/s10681-007-9609-x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

After crosses of multiple rape-radish chromosome additions with rape, nine different monosomic additions (2n = 4x = 38 + 1) of individual radish chromosomes, a-i, were isolated, having the genomic background of winter oil seed rape and radish cytoplasm. Extra chromosomes were identified with RAPD markers, specific for individual radish chromosomes. All radish-plasmic monosomic additions, except that of chromosome f, had low seed production due to pistilloid stamens. Rape cytoplasm was substituted by crossing the monosomic additions as pollinators to rape followed by reselection of the radish chromosome additions. The monosomic additions in rape cytoplasm showed normal male fertility. Average transmission rates of radish chromosomes via egg cells and pollen cells were 37% and 27%, respectively. Lowest male transmission was found for chromosomes e and f with 0.04 and 0.01. Monosomic additions were self-pollinated to produce disomic additions. Monosomic and disomic addition progenies were discriminated by a prescreening with quantitative double primer (dp) RAPDs and cytological confirmation of preselected candidates by FISH analysis using a Raphanus-specific probe. Usability of this complete set of nine disomic rape-radish additions (2n = 4x = 38 + 2) was discussed.

引用

页码：117 / 128

页数：12

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