Preparative separation of cichoric acid from Echinacea Purpurea by pH-zone-refining counter-current chromatography

被引：43

作者：

Wang, X ^{[1
]}

Geng, YL ^{[1
]}

Li, FW ^{[1
]}

Gao, QS ^{[1
]}

Shi, XG ^{[1
]}

机构：

[1] Shandong Acad Sci, Shandong Anal & Test Ctr, Shandong 250014, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1103卷 / 01期

关键词：

Echinacea purpurea; counter-current chromatography; cichoric acid; preparative chromatography;

D O I：

10.1016/j.chroma.2005.11.092

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

pH-zone-refining counter-current chromatography was successfully applied to the separation of cichoric acid from Echinacea Purpurea (L.) Moench. A 3.0 g quantity of sample was separated using the following two-phase solvent system: MtBE-CH3CN-water (4:1:5, v/v), 10 mm trifluoroacetic acid in organic stationary phase and 10 mM ammonia in aqueous mobile phase. The obtained fractions were analyzed by high-performance liquid chromatography and negative ion electrospray ionization mass spectrometry. Double separations were performed with the same solvent system yielding 563 mg cichoric acid at 95.6% purity. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：166 / 169

页数：4

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