Photochemical surface patterning by the thiol-ene reaction

被引：161

作者：

Jonkheijm, Pascal ^{[1
,2
,3
]}

Weinrich, Dirk ^{[1
,2
,3
]}

Koehn, Maja ^{[1
,2
,3
]}

Engelkamp, Hans ^{[4
]}

Christianen, Peter C. M. ^{[4
]}

Kuhlmann, Juergen ^{[1
,2
,3
]}

Maan, Jan C. ^{[4
]}

Nuesse, Dirk ^{[1
]}

Schroeder, Hendrik ^{[1
]}

Wacker, Ron ^{[1
]}

Breinbauer, Rolf ^{[1
,2
,3
]}

Niemeyer, Christof M. ^{[1
]}

Waldmann, Herbert ^{[1
,2
,3
]}

机构：

[1] Tech Univ Dortmund, Fac Chem, D-44227 Dortmund, Germany

[2] Max Planck Inst Mol Physiol, Dept Biol Chem, D-44227 Dortmund, Germany

[3] Max Planck Inst Mol Physiol, Dept Biol Struct, D-44227 Dortmund, Germany

[4] Radboud Univ Nijmegen, Inst Mol & Mat, NL-6525 ED Nijmegen, Netherlands

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 23期

关键词：

fluorescence; immobilization; proteins; surface patterning; thiol-ene reactions;

D O I：

10.1002/anie.200800101

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The photochemical coupling of olefin-capped (bio)molecules to surface-bound thiols can be used to control protein immobilization on length scales of centimeters to sub-micrometers (see the fluorescence microscopy image of a nanopattern after treatment with labeled streptavidin). Two enzymes immobilized on the resulting patterns retained their enzymatic activity and underwent protein-protein interactions similar to those in the solution phase. (Figure Presented). © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：4421 / 4424

页数：4

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