Filamentous, Mixed Micelles of Triblock Copolymers Enhance Tumor Localization of Indocyanine Green in a Murine Xenograft Model

被引：42
作者：
Kim, Tae Hee [1 ]
Mount, Christopher W. [1 ]
Dulken, Benjamin W. [1 ]
Ramos, Jenelyn [3 ]
Fu, Caroline J. [3 ]
Khant, Htet A. [3 ]
Chiu, Wah [3 ]
Gombotz, Wayne R. [2 ]
Pun, Suzie H. [1 ]
机构：
[1] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA
[2] Omeros Corp, Seattle, WA 98101 USA
[3] Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Natl Ctr Macromol Imaging, Houston, TX 77030 USA
来源：
MOLECULAR PHARMACEUTICS | 2012年 / 9卷 / 01期
关键词：
filamentous micelles; indocyanine green; tumor delivery; triblock copolymers; BIODEGRADABLE AMPHIPHILIC POLY(ETHYLENE; DRUG-DELIVERY; BLOCK-COPOLYMERS; DIBLOCK COPOLYMERS; AQUEOUS-SOLUTION; MICELLIZATION PHENOMENA; MULTIDRUG-RESISTANCE; POLYMERIC MICELLES; DOXORUBICIN; OXIDE);
D O I：
10.1021/mp200381c
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Polymeric micelles formed by the self-assembly of amphiphilic block copolymers can be used to encapsulate hydrophobic drugs for tumor-delivery applications. Filamentous carriers with high aspect ratios offer potential advantages over spherical carriers, including prolonged circulation times. In this work, mixed micelles composed of poly(ethylene oxide)-poly[(R)-3-hydroxybutyrate]-poly(ethylene oxide) (PEO-PHB-PEO) and Pluronic F-127 (PF-127) were used to encapsulate a near-infrared fluorophore. The micelle formulations were assessed for tumor accumulation after tail vein injection to xenograft tumor-bearing mice by noninvasive optical imaging. The mixed micelle formulation that facilitated the highest tumor accumulation was shown by cryo-electron microscopy to be filamentous in structure compared to spherical structures of pure PF-127 micelles. In addition, increased dye loading efficiency and dye stability were attained in this mixed micelle formulation compared to pure PEO-PHB-PEO micelles. Therefore, the optimized PEO-PHB-PEO/PF-127 mixed micelle formulation offers advantages for cancer delivery over micelles formed from the individual copolymer components.
引用
收藏
页码：135 / 143
页数：9
相关论文
共 48 条
[21]   Evaluation of Temperature-Sensitive, Indocyanine Green-Encapsulating Micelles for Noninvasive Near-Infrared Tumor Imaging [J].
Kim, Tae Hee ;
Chen, Yongping ;
Mount, Christopher W. ;
Gombotz, Wayne R. ;
Li, Xingde ;
Pun, Suzie H. .
PHARMACEUTICAL RESEARCH, 2010, 27 (09) :1900-1913
[22]   The delivery of doxorubicin to 3-D multicellular spheroids and tumors in a murine xenograft model using tumor-penetrating triblock polymeric micelles [J].
Kim, Tae-Hee ;
Mount, Christopher W. ;
Gombotz, Wayne R. ;
Pun, Suzie H. .
BIOMATERIALS, 2010, 31 (28) :7386-7397
[23]   Stabilization of Indocyanine Green by Encapsulation within Micellar Systems [J].
Kirchherr, Anne-Katrin ;
Briel, Andreas ;
Maeder, Karsten .
MOLECULAR PHARMACEUTICS, 2009, 6 (02) :480-491
[24]   Multidrug resistance (MDR) in cancer - Mechanisms, reversal using modulators of MDR and the role of MDR modulators in influencing the pharmacokinetics of anticancer drugs [J].
Krishna, R ;
Mayer, LD .
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2000, 11 (04) :265-283
[25]   ENHANCED TUMOR ACCUMULATION AND PROLONGED CIRCULATION TIMES OF MICELLE-FORMING POLY(ETHYLENE OXIDE-ASPARTATE) BLOCK COPOLYMER-ADRIAMYCIN CONJUGATES [J].
KWON, G ;
SUWA, S ;
YOKOYAMA, M ;
OKANO, T ;
SAKURAI, Y ;
KATAOKA, K .
JOURNAL OF CONTROLLED RELEASE, 1994, 29 (1-2) :17-23
[26]   Polymeric micelle for tumor pH and folate-mediated targeting [J].
Lee, ES ;
Na, K ;
Bae, YH .
JOURNAL OF CONTROLLED RELEASE, 2003, 91 (1-2) :103-113
[27]   Micellization phenomena of biodegradable amphiphilic triblock copolymers consisting of poly(β-hydroxyalkanoic acid) and poly(ethylene oxide) [J].
Li, J ;
Ni, XP ;
Li, X ;
Tan, NK ;
Lim, CT ;
Ramakrishna, S ;
Leong, KW .
LANGMUIR, 2005, 21 (19) :8681-8685
[28]   Synthesis and characterization of new biodegradable amphiphilic poly(ethylene oxide)-b-poly[(R)-3-hydroxy butyratel-b-poly(ethylene oxide) triblock copolymers [J].
Li, J ;
Li, X ;
Ni, XP ;
Leong, KW .
MACROMOLECULES, 2003, 36 (08) :2661-2667
[29]   Dynamic and static light scattering studies on self-aggregation behavior of biodegradable amphiphilic poly(ethylene oxide)-poly [(R)-3-hydroxybutyrate]-poly(ethylene oxide) triblock copolymers in aqueous solution [J].
Li, X ;
Mya, KY ;
Ni, XP ;
He, CB ;
Leong, KW ;
Li, J .
JOURNAL OF PHYSICAL CHEMISTRY B, 2006, 110 (12) :5920-5926
[30]   Self-assembly of mixed amphiphilic triblock copolymers in aqueous solution [J].
Liu, TB ;
Nace, VM ;
Chu, B .
LANGMUIR, 1999, 15 (09) :3109-3117
12345