Filamentous, Mixed Micelles of Triblock Copolymers Enhance Tumor Localization of Indocyanine Green in a Murine Xenograft Model

被引：42

作者：

Kim, Tae Hee ^{[1
]}

Mount, Christopher W. ^{[1
]}

Dulken, Benjamin W. ^{[1
]}

Ramos, Jenelyn ^{[3
]}

Fu, Caroline J. ^{[3
]}

Khant, Htet A. ^{[3
]}

Chiu, Wah ^{[3
]}

Gombotz, Wayne R. ^{[2
]}

Pun, Suzie H. ^{[1
]}

机构：

[1] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA

[2] Omeros Corp, Seattle, WA 98101 USA

[3] Baylor Coll Med, Verna & Marrs McLean Dept Biochem & Mol Biol, Natl Ctr Macromol Imaging, Houston, TX 77030 USA

来源：

MOLECULAR PHARMACEUTICS | 2012年 / 9卷 / 01期

关键词：

filamentous micelles; indocyanine green; tumor delivery; triblock copolymers; BIODEGRADABLE AMPHIPHILIC POLY(ETHYLENE; DRUG-DELIVERY; BLOCK-COPOLYMERS; DIBLOCK COPOLYMERS; AQUEOUS-SOLUTION; MICELLIZATION PHENOMENA; MULTIDRUG-RESISTANCE; POLYMERIC MICELLES; DOXORUBICIN; OXIDE);

D O I：

10.1021/mp200381c

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Polymeric micelles formed by the self-assembly of amphiphilic block copolymers can be used to encapsulate hydrophobic drugs for tumor-delivery applications. Filamentous carriers with high aspect ratios offer potential advantages over spherical carriers, including prolonged circulation times. In this work, mixed micelles composed of poly(ethylene oxide)-poly[(R)-3-hydroxybutyrate]-poly(ethylene oxide) (PEO-PHB-PEO) and Pluronic F-127 (PF-127) were used to encapsulate a near-infrared fluorophore. The micelle formulations were assessed for tumor accumulation after tail vein injection to xenograft tumor-bearing mice by noninvasive optical imaging. The mixed micelle formulation that facilitated the highest tumor accumulation was shown by cryo-electron microscopy to be filamentous in structure compared to spherical structures of pure PF-127 micelles. In addition, increased dye loading efficiency and dye stability were attained in this mixed micelle formulation compared to pure PEO-PHB-PEO micelles. Therefore, the optimized PEO-PHB-PEO/PF-127 mixed micelle formulation offers advantages for cancer delivery over micelles formed from the individual copolymer components.

引用

页码：135 / 143

页数：9

共 48 条

[1] Block copolymer-based formulation of doxorubicin. From cell screen to clinical trials [J].