Cost-Effective Pooling of DNA from Nasopharyngeal Swab Samples for Large-Scale Detection of Bacteria by Real-Time PCR

被引：23

作者：

Edouard, Sophie ^{[1
]}

Prudent, Elsa ^{[1
]}

Gautret, Philippe ^{[1
]}

Memish, Ziad A. ^{[2
]}

Raoult, Didier ^{[1
]}

机构：

[1] Aix Marseille Univ, URMITE, CNRS 7278, UM63,IRD198,Inserm 1095, Marseille, France

[2] Saudi Minist Hlth, Riyadh, Saudi Arabia

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 03期

关键词：

POLYMERASE-CHAIN-REACTION; STREPTOCOCCUS-PSEUDOPNEUMONIAE; CHLAMYDIA-TRACHOMATIS; INFECTION; SPECIMENS; VIRUS; STRATEGY;

D O I：

10.1128/JCM.03609-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, 37% fewer RT-PCR tests are needed.

引用

页码：1002 / 1004

页数：3

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