Spatial regulation of the actin cytoskeleton by HSF-1 during aging

被引：36

作者：

Higuchi-Sanabria, Ryo ^{[1
]}

Paul, Joseph W., III ^{[1
]}

Durieux, Jenni ^{[1
]}

Benitez, Camila ^{[1
]}

Frankino, Phillip A. ^{[1
]}

Tronnes, Sarah U. ^{[1
]}

Garcia, Gilberto ^{[1
]}

Daniele, Joseph R. ^{[1
]}

Monshietehadi, Samira ^{[1
]}

Dillin, Andrew ^{[1
,2
]}

机构：

[1] Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Glenn Ctr Aging Res, Berkeley, CA 94720 USA

来源：

MOLECULAR BIOLOGY OF THE CELL | 2018年 / 29卷 / 21期

基金：

美国国家科学基金会;

关键词：

HEAT-SHOCK FACTOR; CAENORHABDITIS-ELEGANS; ALPHA-SYNUCLEIN; QUALITY-CONTROL; THERMOTOLERANCE; PROTEOSTASIS; LONGEVITY; STRESS;

D O I：

10.1091/mbc.E18-06-0362

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

There are many studies suggesting an age-associated decline in the actin cytoskeleton, and this has been adopted as common knowledge in the field of aging biology. However, a direct identification of this phenomenon in aging multicellular organisms has not been performed. Here, we express LifeAct::mRuby in a tissue-specific manner to interrogate cytoskeletal organization as a function of age. We show for the first time in Caenorhabditis elegans that the organization and morphology of the actin cytoskeleton deteriorate at advanced age in the muscles, intestine, and hypodermis. Moreover, hsf-1 is essential for regulating cytoskeletal integrity during aging, so that knockdown of hsf-1 results in premature aging of actin and its overexpression protects actin cytoskeletal integrity in the muscles, the intestine, and the hypodermis. Finally, hsf-1 overexpression in neurons alone is sufficient to protect cytoskeletal integrity in nonneuronal cells.

引用

页码：2522 / 2527

页数：6

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