High-fat feeding period affects gene expression in rat white adipose tissue

被引：32
作者：
Lopez, IP
Milagro, FI
Marti, A
Moreno-Aliaga, MJ
Martinez, JA
De Miguel, C
机构：
[1] Univ Navarra, Dept Bioquim & Biol Mol, E-31080 Pamplona, Spain
[2] Univ Navarra, Dept Physiol & Nutr, E-31080 Pamplona, Spain
来源：
MOLECULAR AND CELLULAR BIOCHEMISTRY | 2005年 / 275卷 / 1-2期
关键词：
adipose tissue; adiponectin; caveolins; dystroglycan; gene expression; haemoglobin; high-fat diet; obesity; RMT-7;
D O I：
10.1007/s11010-005-1082-z
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The expression of 76 sequences, previously isolated as differentially expressed in visceral white adipose tissue (WAT) of female rats, fed with a high-fat diet for 11 days (Lopez et al., Biochem Biophys Res Comm 318: 234-239, 2004), was analyzed in epidydimal WAT of male rats after a feeding period of 65 days with the same diet, using microarray technology. After Northern blot validation of the results, only three genes appeared upregulated (caveolin-2, the alpha-1 chain of haemoglobin and rat mammary tumor-7) and two downregulated (adiponectin and dystroglycan). We have also analyzed caveolin-1 gene expression and found that follows the opposite pattern of caveolin-2, indicating that they are inversely regulated. Our results suggest that if feeding with a high-fat diet is prolonged, many of the initial changes in gene expression, probably aimed to consume the energy surplus and prevent excessive fat deposition, are not maintained, and adaptation to an increased lipid storage is developed.
引用
收藏
页码：109 / 115
页数：7
相关论文
共 34 条
[1]   Decrease in serum adiponectin level due to obesity and visceral fat accumulation in children [J].
Asayama, K ;
Hayashibe, H ;
Dobashi, K ;
Uchida, N ;
Nakane, T ;
Kodera, K ;
Shirahata, A ;
Taniyama, M .
OBESITY RESEARCH, 2003, 11 (09) :1072-1079
[2]   The epidemic of obesity and changes in food intake: the Fluoride Hypothesis [J].
Bray, GA .
PHYSIOLOGY & BEHAVIOR, 2004, 82 (01) :115-121
[3]   Will fatty worms help cure human obesity? [J].
Chiang, SH ;
MacDougald, OA .
TRENDS IN GENETICS, 2003, 19 (10) :523-525
[4]   Identification of cathepsin K as a novel marker of adiposity in white adipose tissue [J].
Chiellini, C ;
Costa, M ;
Novelli, SE ;
Amri, EZ ;
Benzi, L ;
Bertacca, A ;
Cohen, P ;
Del Prato, S ;
Friedman, JM ;
Maffei, M .
JOURNAL OF CELLULAR PHYSIOLOGY, 2003, 195 (02) :309-321
[5]   Role of caveolin and caveolae in insulin signaling and diabetes [J].
Cohen, AW ;
Combs, TP ;
Scherer, PE ;
Lisanti, MP .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2003, 285 (06) :E1151-E1160
[6]   Caveolin-1-deficient mice show insulin resistance and defective insulin receptor protein expression in adipose tissue [J].
Cohen, AW ;
Razani, B ;
Wang, XB ;
Combs, TP ;
Williams, TM ;
Scherer, PE ;
Lisanti, MP .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2003, 285 (01) :C222-C235
[7]   Identification of novel genes differentially expressed in omental fat of obese subjects and obese type 2 diabetic patients [J].
Corominola, H ;
Conner, LJ ;
Beavers, LS ;
Gadski, RA ;
Johnson, D ;
Caro, JF ;
Rafaeloff-Phail, R .
DIABETES, 2001, 50 (12) :2822-2830
[8]  
Das M, 2004, Obes Rev, V5, P13, DOI 10.1111/j.1467-789X.2004.00115.x
[9]   Obesity in BSB mice is correlated with expression of genes for iron homeostasis and leptin [J].
Farahani, P ;
Chiu, S ;
Bowlus, CL ;
Boffelli, D ;
Lee, E ;
Fisler, JS ;
Krauss, RM ;
Warden, CH .
OBESITY RESEARCH, 2004, 12 (02) :191-204
[10]   Proteolytic cleavage product of 30-kDa adipocyte complement-related protein increases fatty acid oxidation in muscle and causes weight loss in mice [J].
Fruebis, J ;
Tsao, TS ;
Javorschi, S ;
Ebbets-Reed, D ;
Erickson, MRS ;
Yen, FT ;
Bihain, BE ;
Lodish, HF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (04) :2005-2010
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