Proteins in leaked amniotic fluid as biomarkers diagnostic for prelabor rupture of membranes

被引：12

作者：

Wang, Tao ^{[1
]}

Zhou, Rong ^{[2
]}

Zhang, Lin ^{[1
]}

Wang, Yanyun ^{[1
]}

Song, Chang Ping ^{[1
]}

Lin, Wei ^{[2
]}

Niu, Xiaoyu ^{[2
]}

Lin, Yong ^{[1
,2
]}

Hu, Huaizhong ^{[1
,2
]}

机构：

[1] Sichuan Univ, W China Univ Hosp 2, Lab Mol & Translat Med, Chengdu 610041, Sichuan, Peoples R China

[2] Sichuan Univ, W China Univ Hosp 2, Dept Obstet & Gynecol, Chengdu 610041, Sichuan, Peoples R China

来源：

PROTEOMICS CLINICAL APPLICATIONS | 2011年 / 5卷 / 7-8期

关键词：

Amniotic fluid; Axl; IGFBP-1; Prelabor rupture of membrane; sICAM-1; FACTOR BINDING PROTEIN-1; PREMATURE RUPTURE; FETAL MEMBRANES; INDIGO CARMINE; VAGINAL FLUID; FERN TEST; IMMUNOASSAY; PH; IDENTIFICATION; INJECTION;

D O I：

10.1002/prca.201000123

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Purpose: Early diagnosis of prelabor rupture of membranes (PROM) is essential to protect mother and fetus from intra-uterus infection and preterm birth. A simple and rapid bedside test would help clinicians confirm the diagnosis for early treatment. Experimental design: A protein array was used to screen cervical-vaginal fluid (CVF) and amniotic fluid (AF) samples collected from normal and PROM pregnant women. Enzyme-linked immunosorbent assay was used to quantify two novel and potentially useful analytes, soluble intercellular adhesion molecule-1 (sICAM-1) and Axl receptor tyrosine kinase (Axl). Results: The mean concentration of sICAM-1 and Axl was 85 and 482 times higher separately in 30 healthy AF samples than in 110 CVF samples of normal pregnancies. Comparing 110 CVF samples of PROM/Preterm PROM with 110 CVF samples of normal pregnancies, the diagnostic value for PROM was demonstrated by their high sensitivity and specificity (96.4 and 92.7%, respectively, for sICAM-1, and 92.4 and 90.4%, respectively, for Axl). Conclusions and clinical relevance: The results indicate that sICAM-1 and Axl in AF leaked to vagina are sensitive and specific biomarkers for the diagnosis of PROM. Furthermore, sICAM-1 or Axl can be developed into a rapid strip test for bedside use.

引用

页码：415 / 421

页数：7

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