LEAFY COTYLEDON1, FUSCA3 expression and auxin treatment in relation to somatic embryogenesis induction in Arabidopsis

被引：40
|
作者：
Ledwon, Agnieszka [1 ]
Gaj, Malgorzata D. [1 ]
机构：
[1] Univ Silesia, Dept Genet, PL-40032 Katowice, Poland
来源：
PLANT GROWTH REGULATION | 2011年 / 65卷 / 01期
关键词：
Auxin; Embryogenic transition; FUSCA3; Hormonal mutants; LEAFY COTYLEDON1; LEC2; overexpression; TO-EMBRYONIC TRANSITION; STORAGE PROTEIN GENES; TRANSCRIPTION FACTOR; LEAFY-COTYLEDON1-LIKE GENE; DEVELOPMENTAL CONTROL; REGULATORY NETWORK; ECTOPIC EXPRESSION; HELIANTHUS-ANNUUS; THALIANA; IDENTIFICATION;
D O I：
10.1007/s10725-011-9585-y
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The expression pattern of the LEC1 and FUS3 genes during somatic embryogenesis in Arabidopsis explants (immature zygotic embryos) induced in vitro was analysed, using Real-time quantitative PCR (qRT-PCR). The analysis revealed differential expression of LEC1 but not FUS3 within a 30 day time course of somatic embryo development, and a significant auxin-dependent upregulation of LEC1 was found over the time course. In contrast to embryogenic culture, the level of LEC1 and FUS3 expression was noticeably lower in non-embryogenic callus of Col-0 and hormonal mutants (cbp20 and axr4-1) with low SE-efficiency. In addition, the expression profile of LEC1 and FUS3 was followed in the embryogenic culture derived from 35S::LEC2-GR explants. A significant increase of LEC1 but not FUS3 activity was observed under LEC2 overexpression induced in auxin-treated explants. The work provides further experimental evidence on LEC gene involvement in the embryogenic response in Arabidopsis somatic cells, and also implicates LEC1 function in more advanced stages of SE culture in relation to somatic embryo differentiation and development.
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收藏
页码：157 / 167
页数：11
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