Electrochemical Studies of a Novel Biosensor Based on the CuO Nanoparticles Coated with Horseradish Peroxidase to Determine the Concentration of Phenolic Compounds

被引：13

作者：

Kazemi, S. H. ^{[1
]}

Khajeh, K. ^{[2
]}

机构：

[1] IASBS, Dept Chem, Zanjan 4513766731, Iran

[2] Tarbiat Modares Univ, Fac Biol Sci, Tehran, Iran

来源：

JOURNAL OF THE IRANIAN CHEMICAL SOCIETY | 2011年 / 8卷

关键词：

Phenolic compounds; Peroxidase; Nanoparticles; Biosensor; Electrochemical impedance; SELF-ASSEMBLED MONOLAYERS; AMPEROMETRIC BIOSENSOR; POLYPHENOLS; TYROSINASE; FILMS; IMMOBILIZATION; NANOSTRUCTURES; ELECTRODES; SURFACE;

D O I：

10.1007/BF03254292

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The present work is an attempt to design new electrochemical biosensor that easily determines the concentration of phenolic compounds in water and wastewaters. It is well-known that enzymes can add selectivity towards one or limited number of compounds. Thus, in the present study, horseradish peroxidase (HRP) was coated on CuO nanoparticles and immobilized on the electrode using a thin polymeric film. The electrochemical studies of modified electrode were carried out by using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The voltammetric and EIS results confirm the possibility of HRP direct electron transfer at CuO nanoparticles. Our results revealed that the modified electrode can be used to determine the phenol concentration in the standard solutions and real samples. The present biosensor has good relative standard deviation (RSD% = 7%) and detection limit (DL of 0.1 mu M), in addition to high stability.

引用

页码：S152 / S160

页数：9

共 26 条

[1] Amplification of amperometric biosensor responses by electrochemical substrate recycling.: 3.: Theoretical and experimental study of the phenol-polyphenol oxidase system immobilized in Laponite hydrogels and layer-by-layer self-assembled structures [J].