Determination of DNA in human urine by fluorescence probe of acridine orange

被引：0

作者：

Wang, JW ^{[1
]}

Zhu, XP ^{[1
]}

Sun, W ^{[1
]}

Xue, JG ^{[1
]}

Wang, ZX ^{[1
]}

Fu, MG ^{[1
]}

机构：

[1] Nanchang Univ, Dept Chem, Nanchang 330047, Peoples R China

来源：

SPECTROSCOPY AND SPECTRAL ANALYSIS | 2003年 / 23卷 / 05期

关键词：

deoxyribonucleic acid; acridine orange; fluorescence method; human urine;

D O I：

暂无

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Acridine orange has an equilibrium of aggregate forms in the presence of dodecylbenzene sulfonic acid sodium salt, but the equilibrium can shift when DNA is added to the system, thus leading to the enhancement of fluorescence intensity. The intensity shows a good linear relation with the concentration of the DNA in a certain range. Therefore, a new highly sensitive method for the determination of trace amount of DNA was developped. The maximum excitation and emission wavelengths are 506 and 529 nm, respectively, and the excitation slit and emission slit are both 5 nm. For the fish sperm DNA, the linear range of the determination is 0-10 mug.mL(-1), and the determination limit is 0.088 mug.mL(-1). The method has been successfully applied to the clinic determination of DNA in human urine.

引用

页码：899 / 902

页数：4

共 8 条

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