Determination of Pilocarpine in Human Plasma by LC-APCI-MS-MS and Application to a Pharmacokinetic Study

被引：4
|
作者：
Yu, Peng [1 ]
Yang, Guoping [2 ]
Tan, Hongyi [2 ]
Cheng, Zeneng [1 ]
Song, Ming [1 ]
Gu, Zhenkun [1 ]
Li, Xiao [1 ]
机构：
[1] Cent S Univ, Sch Pharmaceut Sci, Changsha 410013, Hunan, Peoples R China
[2] Cent S Univ, Xiangya Hosp 3, Changsha 410013, Hunan, Peoples R China
来源：
CHROMATOGRAPHIA | 2011年 / 73卷 / 9-10期
关键词：
Column liquid chromatography; LC-APCI-MS-MS; Pilocarpine; Human plasma; Pharmacokinetic study; ORAL PILOCARPINE; POSTIRRADIATION XEROSTOMIA; NECK-CANCER; HEAD; MULTICENTER; EFFICACY; URINE;
D O I：
10.1007/s10337-011-1928-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive, specific and rapid high performance liquid chromatography-atmospheric pressure chemical ionization source-tandem mass spectrometry (LC-APCI-MS-MS) method for the determination of pilocarpine in human plasma was developed and validated. The method is based on liquid-liquid extraction, followed by a reversed-phase liquid chromatographic separation, and detected by means of tandem mass spectrometry. The linear calibration curve covered a concentration range of 2-500 mu g L-1. The intra- and inter-day precisions for pilocarpine were < 10% and the accuracies were between 90 and 110%. The method was applied successfully to a pharmacokinetic study involving 20 healthy Chinese male volunteers after oral administration of 6 mg pilocarpine.
引用
收藏
页码：921 / 927
页数：7
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