Molecular detection and identification of Anaplasma and Ehrlichia species in cattle from Turkey

被引：79
作者：
Aktas, Munir [1 ]
Altay, Kursat [1 ]
Dumanli, Nazir [1 ]
机构：
[1] Firat Univ, Dept Parasitol, Fac Vet Med, TR-23119 Elazig, Turkey
来源：
TICKS AND TICK-BORNE DISEASES | 2011年 / 2卷 / 01期
关键词：
Anaplasma; Ehrlichia; PCR; RLB; Cattle; GENETIC DIVERSITY; NATURAL-HISTORY; FARM-ANIMALS; TICK; THEILERIA; BABESIA; INFECTIONS; MARGINALE; PHAGOCYTOPHILA; ORGANISMS;
D O I：
10.1016/j.ttbdis.2010.11.002
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Bovine anaplasmosis is a tick-borne rickettsial disease widespread in tropical and subtropical areas. We investigated the presence and distribution of Anaplasma spp. in cattle from 6 provinces in Turkey. For amplification of the segment spanning the V1 region of the 165 ribosomal RNA (rRNA) gene of Anaplasma species, a reverse line blot (RIB) hybridization assay was performed on 389 blood samples. RLB identified Anaplasma infections in 9.0% (35/389) of the samples. The most frequently found species was A. marginale (11/389, 2.8%), followed by A. centrale (4/389, 1.0%) and A. phagocytophilum (4/389, 1.0%). Eighteen of 35 PCR-positive samples gave positive signals to the catch-all probes, but did not show any response to the species-specific probes tested. Sequencing results of 5 representative amplicons randomly selected from these specimens indicated that 3 were 100% identical to the sequence of A. ovis, and the other 2 sequences were 99.5% identical to the sequence of Ehrlichia sp. Omatjenne strain. The results further confirmed that A. ovis and Ehrlichia sp. Omatjenne infection occurs in cattle populations in Turkey. (C) 2010 Elsevier GmbH. All rights reserved.
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收藏
页码：62 / 65
页数：4
相关论文
共 36 条
[21]   Anaplasma marginale (Rickettsiales: Anaplasmataceae):: recent advances in defining host-pathogen adaptations of a tick-borne rickettsia [J].
Kocan, KM ;
De La Fuente, J ;
Blouin, EF ;
Garcia-Garcia, JC .
PARASITOLOGY, 2004, 129 :S285-S300
[22]   IMMUNOSUPPRESSION IN SHEEP EXPERIMENTALLY INFECTED WITH EHRLICHIA-PHAGOCYTOPHILA [J].
LARSEN, HJS ;
OVERNES, G ;
WALDELAND, H ;
JOHANSEN, GM .
RESEARCH IN VETERINARY SCIENCE, 1994, 56 (02) :216-224
[23]   Molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in South Africa [J].
Matjila, Paul Tshepo ;
Leisewitz, Andrew L. ;
Jongejan, Frans ;
Penzhorn, Barend L. .
VETERINARY PARASITOLOGY, 2008, 155 (1-2) :152-157
[24]   Babesia bicornis sp nov and Theileria bicornis sp nov.:: Tick-borne parasites associated with mortality in the black rhinoceros (Diceros bicornis) [J].
Nijhof, AM ;
Penzhorn, BL ;
Lynen, G ;
Mollel, JO ;
Morkel, P ;
Bekker, CPJ ;
Jongejan, F .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (05) :2249-2254
[25]   Field evidence for density-dependent facilitation amongst Ixodes ricinus ticks feeding on sheep . [J].
Ogden, NH ;
Casey, ANJ ;
French, NP ;
Adams, JDW ;
Woldehiwet, Z .
PARASITOLOGY, 2002, 124 :117-125
[26]   Anaplasma marginale infection in a Japanese Black cow 13 years after eradication of Rhipicephalus (Boophilus) microplus in Okinawa, Japan [J].
Ooshiro, Mamoru ;
Zakimi, Satoshi ;
Matsukawa, Yoshimasa ;
Yafuso, Makoto ;
Katagiri, Yoshito ;
Inokuma, Hisashi .
VETERINARY PARASITOLOGY, 2009, 160 (3-4) :351-355
[27]  
Potgieter F.T., 1994, Infectious Diseases of Livestock-With Special Reference to Southern Africa, P408
[28]   Genetic diversity of Anaplasma marginale in Argentina [J].
Ruybal, Paula ;
Moretta, Rosalia ;
Perez, Andres ;
Petrigh, Romina ;
Zimmer, Patricia ;
Alcaraz, Elda ;
Echaide, Ignacio ;
de Echaide, Susana Torioni ;
Kocan, Katherine M. ;
de la Fuente, Jose ;
Farber, Marisa .
VETERINARY PARASITOLOGY, 2009, 162 (1-2) :176-180
[29]   Detection and identification of Ehrlichia, Borrelia burgdorferi sensu lato, and Bartonella species in Dutch Ixodes ricinus ticks [J].
Schouls, LM ;
Van de Pol, I ;
Rijpkema, SGT ;
Schot, CS .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (07) :2215-2222
[30]   Identification of Anaplasma phagocytophila (formerly Ehrlichia phagocytophila) variants in blood from sheep in Norway [J].
Stuen, S ;
Van de Pol, I ;
Bergström, K ;
Sehouls, LA .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (09) :3192-3197
1234