Chiral analysis of derivatized amino acids from kefir by gas chromatography

被引:20
作者
Menestrina, Fiorella
Osorio Grisales, Jaiver
Castells, Cecilia B. [1 ]
机构
[1] UNLP, CONICET, LIDMA, 47 & 115 B1900AJL, La Plata, Buenos Aires, Argentina
关键词
Gas chromatography; Chiral; Amino acids analysis; Kefir; MASS-SPECTROMETRY; ENANTIOMERS; PHASE; QUANTIFICATION;
D O I
10.1016/j.microc.2016.05.007
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The amino acids present in the kefir samples were derivatized using different alkyl chloroformates combined with alcohols and chromatographed on a (3-O-butanoyl-2,6-di-O-pentyl)-gamma-cyclodextrin (Lipodex E) chiral gas-liquid chromatographic column. The combination of derivatization reagents that led to better chromatographic performance for standard amino acid solutions was critically compared. All the chloroformate/alchohol mixtures lead to fast and quantitative reactions. However, the ethyl chloroformate/ethanol mixture was selected since the number of enantioseparated amino acid derivatives was higher. The derivatization was applied to samples of kefir grains grown in raw and in skimmed UHT milk. The samples were previously pretreated by elution from a low-pressure ion-exchange columns. Standard addition calibration curves have been used for the quantitative determinations of amino acid derivatives in both samples, and the figs. of merit of the proposed method have been assessed. The L- and n- configuration of o- and L-alanine, D- and L-valine, o-proline, L-threonine, aspartic and glutamic acids, methionine, and cysteine were detected and quantitatively determined. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:267 / 273
页数:7
相关论文
共 24 条
[1]   Rapid analysis of amino acid enantiomers by chiral-phase capillary gas chromatography [J].
Abe, I ;
Fujimoto, N ;
Nishiyama, T ;
Terada, K ;
Nakahara, T .
JOURNAL OF CHROMATOGRAPHY A, 1996, 722 (1-2) :221-227
[2]   GAS-CHROMATOGRAPHIC DETECTION OF D-AMINO ACIDS AS COMMON CONSTITUENTS OF FERMENTED FOODS [J].
BRUCKNER, H ;
HAUSCH, M .
CHROMATOGRAPHIA, 1989, 28 (9-10) :487-492
[3]   CHIRALITY OF AMINO-ACIDS OF MICROORGANISMS USED IN FOOD BIOTECHNOLOGY [J].
BRUCKNER, H ;
BECKER, D ;
LUPKE, M .
CHIRALITY, 1993, 5 (05) :385-392
[4]   Gas chromatographic quantification of amino acid enantiomers in food matrices by their N(O,S)-ethoxycarbonyl heptafluorobutyl ester derivatives [J].
Casal, S ;
Oliveira, MB ;
Ferreira, MA .
JOURNAL OF CHROMATOGRAPHY A, 2000, 866 (02) :221-230
[5]   Relationship among Plasma Amino Acids, C-Reactive Protein, Illness Severity, and Outcome in Critically Ill Dogs [J].
Chan, D. L. ;
Rozanski, E. A. ;
Freeman, L. M. .
JOURNAL OF VETERINARY INTERNAL MEDICINE, 2009, 23 (03) :559-563
[6]   Microbiological and chemical properties of kefir manufactured by entrapped microorganisms isolated from kefir grains [J].
Chen, T. -H. ;
Wang, S. -Y. ;
Chen, K. -N. ;
Liu, J. -R. ;
Chen, M. -J. .
JOURNAL OF DAIRY SCIENCE, 2009, 92 (07) :3002-3013
[7]   NOMENCLATURE IN EVALUATION OF ANALYTICAL METHODS INCLUDING DETECTION AND QUANTIFICATION CAPABILITIES (IUPAC RECOMMENDATIONS 1995) [J].
CURRIE, LA .
PURE AND APPLIED CHEMISTRY, 1995, 67 (10) :1699-1723
[8]   ENANTIOMER LABELING, A METHOD FOR THE QUANTITATIVE-ANALYSIS OF AMINO-ACIDS [J].
FRANK, H ;
NICHOLSON, GJ ;
BAYER, E .
JOURNAL OF CHROMATOGRAPHY, 1978, 167 (DEC) :187-196
[9]   RAPID GAS-CHROMATOGRAPHIC SEPARATION OF AMINO-ACID ENANTIOMERS WITH A NOVEL CHIRAL STATIONARY PHASE [J].
FRANK, H ;
NICHOLSON, GJ ;
BAYER, E .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1977, 15 (05) :174-176
[10]   Origin, Microbiology, Nutrition, and Pharmacology of D-Amino Acids [J].
Friedman, Mendel .
CHEMISTRY & BIODIVERSITY, 2010, 7 (06) :1491-1530