Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture

被引：8

作者：

Shin, Hyun Yong ^{[1
]}

Lee, Jin Young ^{[1
]}

Park, Chulhwan ^{[2
]}

Kim, Seung Wook ^{[1
]}

机构：

[1] Korea Univ, Dept Chem & Biol Engn, Seoul 136701, South Korea

[2] Kwangwoon Univ, Dept Chem Engn, Seoul, South Korea

来源：

JOURNAL OF BIOTECHNOLOGY | 2011年 / 151卷 / 04期

关键词：

Cysteine; Acremonium chrysogenum; Methionine; Cephalosporin C; CYSTATHIONINE-GAMMA-LYASE; PENICILLIN-N; ISOPENICILLIN-N; FATTY-ACIDS; MECB GENE; BIOSYNTHESIS; VALINE; STIMULATION; SYNTHETASE; CONVERSION;

D O I：

10.1016/j.jbiotec.2010.12.021

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, the role of glycerol in cephalosporin C production was evaluated in Acremonium chrysogenum M35. Methionine-unsupplemented culture with glycerol stimulated cephalosporin C production in A. chrysogenum M35 by up to 10-fold while increasing the amount of cysteine. Glycerol caused the upregulation of isopenicillin N-CoA epimerase (cefD2) and delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthase (pcbAB) transcription in exponential phase. These results indicate that glycerol without methionine effectively enhanced cephalosporin C production via stimulation of cysteine and valine in A. chrysogemun M35. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：363 / 368

页数：6

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