Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture

被引：8

作者：

Shin, Hyun Yong ^{[1
]}

Lee, Jin Young ^{[1
]}

Park, Chulhwan ^{[2
]}

Kim, Seung Wook ^{[1
]}

机构：

[1] Korea Univ, Dept Chem & Biol Engn, Seoul 136701, South Korea

[2] Kwangwoon Univ, Dept Chem Engn, Seoul, South Korea

来源：

JOURNAL OF BIOTECHNOLOGY | 2011年 / 151卷 / 04期

关键词：

Cysteine; Acremonium chrysogenum; Methionine; Cephalosporin C; CYSTATHIONINE-GAMMA-LYASE; PENICILLIN-N; ISOPENICILLIN-N; FATTY-ACIDS; MECB GENE; BIOSYNTHESIS; VALINE; STIMULATION; SYNTHETASE; CONVERSION;

D O I：

10.1016/j.jbiotec.2010.12.021

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, the role of glycerol in cephalosporin C production was evaluated in Acremonium chrysogenum M35. Methionine-unsupplemented culture with glycerol stimulated cephalosporin C production in A. chrysogenum M35 by up to 10-fold while increasing the amount of cysteine. Glycerol caused the upregulation of isopenicillin N-CoA epimerase (cefD2) and delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthase (pcbAB) transcription in exponential phase. These results indicate that glycerol without methionine effectively enhanced cephalosporin C production via stimulation of cysteine and valine in A. chrysogemun M35. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：363 / 368

页数：6

共 33 条

[1] ARAKI K, 1991, KIRKOTHMER ENCY CHEM, V9, P504
[2] FURTHER-STUDIES ON CARBON CATABOLITE REGULATION OF BETA-LACTAM ANTIBIOTIC-SYNTHESIS IN CEPHALOSPORIUM-ACREMONIUM
BEHMER, CJ
DEMAIN, AL
[J]. CURRENT MICROBIOLOGY, 1983, 8 (02) : 107 - 114
[3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4] DEMAIN AL, 1987, DEV IND MICROBIOL, V27, P175
[5] REGULATORY PROPERTIES OF O-ACETYL-L-SERINE SULFHYDRYLASE OF CEPHALOSPORIUM-ACREMONIUM - EVIDENCE OF AN ISOENZYME AND ITS IMPORTANCE IN CEPHALOSPORIN-C BIOSYNTHESIS
DOBELI, H
NUESCH, J
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1980, 18 (01) : 111 - 117
[6] A SPECTROPHOTOMETRIC METHOD FOR DIRECT DETERMINATION OF CYSTEINE IN PRESENCE OF OTHER NATURALLY OCCURRING AMINO ACIDS
GAITONDE, MK
[J]. BIOCHEMICAL JOURNAL, 1967, 104 (02) : 627 - &
[7] GEIGER F, 1998, Patent No. 5770769
[8] GUTIERREZ S, 1991, J BACTERIOL, V173, P2354
[9] Isolation and Characterization of Thermotolerant Fungi Producing Lignoceric Acid from Glycerol
Habe, Hiroshi
Morita, Tomotake
Sakaki, Keiji
Kitamoto, Dai
[J]. JOURNAL OF OLEO SCIENCE, 2008, 57 (04) : 251 - 255
[10] CPCR1, but not its interacting transcription factor AcFKH1, controls fungal arthrospore formation in Acremonium chrysogenum
Hoff, B
Schmitt, EK
Kück, U
[J]. MOLECULAR MICROBIOLOGY, 2005, 56 (05) : 1220 - 1233

← 1 2 3 4 →