Direct measurement of neuronal ensemble activity using photoacoustic imaging in the stimulated Fos-LacZ transgenic rat brain: A proof-of-principle study

被引：18

作者：

Matchynski, James, I ^{[1
,2
,3
]}

Manwar, Rayyan ^{[4
]}

Kratkiewicz, Karl J. ^{[5
]}

Madangopal, Rajtarun ^{[6
]}

Lennon, Veronica A. ^{[6
,7
]}

Makki, Kassem M. ^{[1
]}

Reppen, Abbey L. ^{[1
]}

Woznicki, Alexander R. ^{[1
]}

Hope, Bruce T. ^{[6
]}

Perrine, Shane A. ^{[1
,2
,3
]}

Conti, Alana C. ^{[1
,2
,3
]}

Avanaki, Kamran ^{[4
,8
]}

机构：

[1] John D Dingell Vet Affairs Med Ctr, Detroit, MI USA

[2] Wayne State Univ, Sch Med, Translat Neurosci Program, Detroit, MI USA

[3] Wayne State Univ, Dept Psychiat & Behav Neurosci, Sch Med, Detroit, MI USA

[4] Univ Illinois, Richard & Loan Hill Dept Biomed Engn, Chicago, IL 60607 USA

[5] Wayne State Univ, Dept Biomed Engn, Detroit, MI USA

[6] NIDA, Intramural Res Program, Baltimore, MD USA

[7] Icahn Sch Med Mt Sinai, Nash Family Dept Neurosci, New York, NY 10029 USA

[8] Univ Illinois, Dept Dermatol, Chicago, IL 60680 USA

来源：

PHOTOACOUSTICS | 2021年 / 24卷

基金：

美国国家卫生研究院;

关键词：

Photoacoustic imaging; Neuronal ensemble; Brain; Fos; X-gal; Cocaine; Fear conditioning; MEDIAL PREFRONTAL CORTEX; C-FOS; OPTICAL-PROPERTIES; EXPRESSION; COCAINE; AMYGDALA; SYSTEM; CONNECTIVITY; TOMOGRAPHY; EXTINCTION;

D O I：

10.1016/j.pacs.2021.100297

中图分类号：

O42 [声学];

学科分类号：

070206 ; 082403 ;

摘要：

Measuring neuroactivity underlying complex behaviors facilitates understanding the microcircuitry that supports these behaviors. We have developed a functional and molecular photoacoustic tomography (F/M-PAT) system which allows direct imaging of Fos-expressing neuronal ensembles in Fos-LacZ transgenic rats with a large fieldof-view and high spatial resolution. F/M-PAT measures the beta-galactosidase catalyzed enzymatic product of exogenous chromophore X-gal within ensemble neurons. We used an ex vivo imaging method in the Wistar FosLacZ transgenic rat, to detect neuronal ensembles in medial prefrontal cortex (mPFC) following cocaine administration or a shock-tone paired stimulus. Robust and selective F/M-PAT signal was detected in mPFC neurons after both conditions (compare to naive controls) demonstrating successful and direct detection of Fosexpressing neuronal ensembles using this approach. The results of this study indicate that F/M-PAT can be used in conjunction with Fos-LacZ rats to monitor neuronal ensembles that underlie a range of behavioral processes, such as fear learning or addiction.

引用

页数：13

共 1 条

[1] Quantification of prefrontal cortical neuronal ensembles following conditioned fear learning in a Fos-LacZ transgenic rat with photoacoustic imaging in Vivo
Matchynski, James I.
Cilley, Timothy S.
Sadik, Nareen
Makki, Kassem M.
Wu, Min
Manwar, Rayyan
Woznicki, Alexander R.
Kallakuri, Srinivasu
Arfken, Cynthia L.
Hope, Bruce T.
Avanaki, Kamran
Conti, Alana C.
Perrine, Shane A.
PHOTOACOUSTICS, 2023, 33

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