The Spectrophotometric determination of proteins using zincon

被引：0

作者：

Chi, YH ^{[1
]}

Li, N ^{[1
]}

Zhuang, J ^{[1
]}

Li, KA ^{[1
]}

Tong, SY ^{[1
]}

机构：

[1] Peking Univ, Dept Chem, Beijing 100871, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 1998年 / 19卷 / 06期

关键词：

protein; zincon; spectrophotometry;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

When BSA was added into zincon solution at pH 4.1, the purple colour was observed, which indicates the formation of zincon-BSA complex. The maximum absorption of complex was 564 nm and 594 nm with the apparent molar absorptivity of 1.5 x 10(6) L . mol(-1) . cm(-1). The linear range of standard curve for BSA is 0 similar to 10 mu g/mL, and the detection limit is 0.5 mu g/mL. The basic reaction conditions were investigated. This proposed method was simple, sensitive, and reproducible. The analytical results of human Serum albumin using this method is very satisfactory.

引用

页码：879 / 881

页数：3