Suitability of Nanoparticles to Face Benzo(a)pyrene-Induced Genetic and Chromosomal Damage in M. galloprovincialis. An In Vitro Approach

被引：6

作者：

Bernardeschi, Margherita ^{[1
,2
]}

Guidi, Patrizia ^{[1
,2
]}

Palumbo, Mara ^{[1
,2
]}

Genovese, Massimo ^{[3
]}

Alfe, Michela ^{[4
]}

Gargiulo, Valentina ^{[4
]}

Lucchesi, Paolo ^{[1
,2
]}

Scarcelli, Vittoria ^{[1
,2
]}

Falleni, Alessandra ^{[1
,2
]}

Bergami, Elisa ^{[5
,6
]}

Freyria, Francesca S. ^{[7
]}

Bonelli, Barbara ^{[7
]}

Corsi, Ilaria ^{[5
,6
]}

Frenzilli, Giada ^{[1
,2
]}

机构：

[1] Univ Pisa, Dept Clin & Expt Med, Sect Appl Biol & Genet, I-56126 Pisa, Italy

[2] Univ Pisa, Dept Clin & Expt Med, INSTM Local Unit, I-56126 Pisa, Italy

[3] Univ Florence, Dept Expt & Clin Biomed Sci Mario Serio, I-50121 Florence, Italy

[4] CNR, STEMS, Inst Sci & Technol Sustainable Energy & Mobil, I-80126 Naples, Italy

[5] Univ Siena, Dept Phys, Earth & Environm Sci, I-53100 Siena, Italy

[6] Univ Siena, Dept Phys, INSTM Local Unit, I-53100 Siena, Italy

[7] Politecn Torino, Dept Appl Sci & Technol, INSTM Unit Torino Politecn, I-10129 Turin, Italy

来源：

NANOMATERIALS | 2021年 / 11卷 / 05期

关键词：

DNA damage; chromosomal damage; cytome assay; titanium dioxide nanoparticles; carbon black derived nanoparticles; genotoxicity; cellular uptake; mussel gill biopsy; nanoremediation; benzo(a)pyrene; TITANIUM-DIOXIDE NANOPARTICLES; POLYCYCLIC AROMATIC-HYDROCARBONS; DOLPHIN TURSIOPS-TRUNCATUS; MYTILUS-GALLOPROVINCIALIS; DNA-DAMAGE; GILL CELLS; CARBON NANOTUBES; PRIMARY CULTURES; BENZO(A) PYRENE; QUANTUM DOTS;

D O I：

10.3390/nano11051309

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Benzo(a)pyrene (B(a)P) is a well-known genotoxic agent, the removal of which from environmental matrices is mandatory, necessitating the application of cleaning strategies that are harmless to human and environmental health. The potential application of nanoparticles (NPs) in the remediation of polluted environments is of increasing interest. Here, specifically designed NPs were selected as being non-genotoxic and able to interact with B(a)P, in order to address the genetic and chromosomal damage it produces. A newly formulated pure anatase nano-titanium (nano-TiO2), a commercial mixture of rutile and anatase, and carbon black-derived hydrophilic NPs (HNP) were applied. Once it had been ascertained that the NPs selected for the work did not induce genotoxicity, marine mussel gill biopsies were exposed in vitro to B(a)P (2 mu g/mL), alone and in combination with the selected NPs (50 mu g/mL nano-TiO2, 10 mu g/mL HNP). DNA primary reversible damage was evaluated by means of the Comet assay. Chromosomal persistent damage was assessed on the basis of micronuclei frequency and nuclear abnormalities by means of the Micronucleus-Cytome assay. Transmission Electron Microscopy (TEM) was performed to investigate the mechanism of action exerted by NPs. Pure Anatase n-TiO2 was found to be the most suitable for our purpose, as it is cyto- and genotoxicity free and able to reduce the genetic and chromosomal damage associated with exposure to B(a)P.

引用

页数：18

共 74 条

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