Importance of tissue sampling, laboratory methods, and patient characteristics for detection of Pneumocystis in autopsied lungs of non-immunosuppressed individuals

被引：7

作者：

Vargas, S. L. ^{[1
]}

Ponce, C. ^{[1
]}

Bustamante, R. ^{[1
]}

Calderon, E. ^{[2
,3
]}

Nevez, G. ^{[4
,5
]}

De Armas, Y. ^{[6
]}

Matos, O. ^{[7
]}

Miller, R. F. ^{[8
,9
]}

Gallo, M. J. ^{[10
]}

机构：

[1] Univ Chile, Inst Ciencias Biomed, Fac Med, Programa Microbiol & Micol, Independencia 1027, Santiago 8380453, Chile

[2] Univ Seville, CSIC, Hosp Univ Virgen del Rocio, CIBERESP, Seville, Spain

[3] Univ Seville, CSIC, Hosp Univ Virgen del Rocio, Inst Biomed Sevilla, Seville, Spain

[4] Brest Univ Hosp, Lab Parasitol & Mycol, Brest, France

[5] Univ Brest, GEIHP, EA 3142, Brest, France

[6] Inst Trop Med Pedro Kouri Hosp, Inst Trop Med Pedro Kouri Pathol Dept, Hosp Microbiol Dept, Havana, Cuba

[7] Univ Nova Lisboa, Inst Higiene & Med Trop, Unidade Parasitol Med, Grp Protozoarios Oportunistas VIH & Outros Protoz, P-1349008 Lisbon, Portugal

[8] UCL, Inst Global Hlth, Res Dept Infect & Populat Hlth, Mortimer Market St, London WC1E 6BT, England

[9] London Sch Hyg & Trop Med, Dept Clin Res, London, England

[10] Serv Med Legal, Av La Paz 1012, Santiago 8380454, Chile

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2017年 / 36卷 / 10期

关键词：

F-SP HOMINIS; CARINII; JIROVECII; DNA; COLONIZATION; INFECTION; PNEUMONIA; INFANTS;

D O I：

10.1007/s10096-017-3006-8

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

To understand the epidemiological significance of Pneumocystis detection in a lung tissue sample of non-immunosuppressed individuals, we examined sampling procedures, laboratory methodology, and patient characteristics of autopsy series reported in the literature. Number of tissue specimens, DNA-extraction procedures, age and underlying diagnosis highly influence yield and are critical to understand yield differences of Pneumocystis among reports of pulmonary colonization in immunocompetent individuals.

引用

页码：1711 / 1716

页数：6

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