Engineered production of pyridoxal 5 '-phosphate in Escherichia coli BL21

被引：5

作者：

He, Min ^{[1
]}

Ma, Jian ^{[1
]}

Chen, Qingwei ^{[1
]}

Zhang, Qili ^{[1
]}

Yu, Ping ^{[1
]}

机构：

[1] Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, 149 Jiaogong Rd, Hangzhou 310035, Peoples R China

来源：

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2022年 / 52卷 / 05期

关键词：

Escherichia coli; metabolic engineering; pyridoxal 5 '-phosphate; response surface methodology; VITAMIN-B-6; BIOSYNTHESIS; BIOCHEMICAL-CHARACTERIZATION; SACCHAROMYCES-CEREVISIAE; MOLECULAR EVOLUTION; GENE; SYNTHASE; OPTIMIZATION; ARABIDOPSIS; ENZYMES; 1-DEOXY-D-XYLULOSE-5-PHOSPHATE;

D O I：

10.1080/10826068.2021.1966801

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Pyridoxal 5 '-phosphate (PLP) is the coenzyme of more than 140 enzymes and is widely used in various fields. In this study, to enhance the production of PLP in Escherichia coli BL21, the recombinant strain E. coli BL21/pETDuet-1-pdxj-zwf-dxs was constructed. The concentration of PLP in this strain was 82.69 mg/L, which was increased by 1.38-fold as compared to that in E. coli BL21. Glucose, yeast extract, and pH had an obvious impact on the concentration of PLP, and their optimal levels were 34.89 g/L, 31.17 g/L, and 10.07, respectively. The concentration of PLP under the optimal condition reached 2.23 g/L. The time-course analysis showed that the highest concentration of PLP was 2.32 g/L in recombinant strain after the induction for 12 h by 0.1 mM IPTG in a 1 L shake flask, which was increased by 38.76-fold as compared to that in E. coli BL21. This study provides a good basis for the efficient production of PLP in E. coli BL21.

引用

页码：498 / 507

页数：10

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[41] Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol [J].