Comparative study of Lowry and Bradford methods: interfering substances

被引：61
作者：
Lucarini, AC [1 ]
Kilikian, BV [1 ]
机构：
[1] USP, Escola Politecn, Dept Engn Quim, BR-05424970 Sao Paulo, Brazil
来源：
BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 02期
基金：
巴西圣保罗研究基金会;
关键词：
protein precipitation; ethanol; glucoamylase; Lowry; Bradford; protein determination;
D O I：
10.1023/A:1008995609027
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The methods of Lowry and Bradford, commonly used for protein determination, were compared regarding the level of interference of some substances used for glucoamylase precipitation by ethanol. The method of Bradford suffers no interference while the method of Lowry showed protein concentration values 20% increased in the presence of ethanol and Tris. Despite these interferences, the Lowry method can evaluate more accurately the increase of purity during fractionation, due to its sensitivity to low molecular weight (below 6 kDa) proteins and peptides.
引用
收藏
页码：149 / 154
页数：6
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