Hyperbaric oxygen preconditioning induces tolerance against brain ischemia-reperfusion injury by upregulation of antioxidant enzymes in rats

被引：112

作者：

Li, Jiasi ^{[1
]}

Liu, Wenwu ^{[2
]}

Ding, Suju ^{[1
]}

Xu, Weigang ^{[2
]}

Guan, Yanytai ^{[1
]}

Zhang, John H. ^{[3
]}

Sun, Xuejun ^{[2
]}

机构：

[1] Changhai Hosp, Dept Neurol, Shanghai 200433, Peoples R China

[2] Second Mil Med Univ, Fac Naval Med, Dept Diving Med, Shanghai 200433, Peoples R China

[3] Loma Linda Univ, Dept Neurosurg, Loma Linda, CA 92350 USA

来源：

BRAIN RESEARCH | 2008年 / 1210卷

关键词：

hyperbaric oxygen; prevention; antioxidant enzyme; ischemic tolerance; reactive oxygen species;

D O I：

10.1016/j.brainres.2008.03.007

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The present study examined the hypothesis that cerebral ischemic tolerance induced by hyperbaric oxygen preconditioning (HBO-PC) is associated with an increase of antioxidant enzyme activity. Male Sprague-Dawley rats (250-280 g, n=74) were divided into sham, middle cerebral artery occlusion (MCAO) for 90 min, and MCAO plus HBO-PC groups. HBO-PC was conducted four times by given 100% oxygen at 2.5 atmosphere absolute (ATA), for 1 h at every 12 h interval for 2 days. At 24 h after the last HBO-PC, MCAO was performed and at 24 h after MCAO, neurological function and Nissl Staining were performed to evaluate the effect of HBO-PC. Malondialdehyde (MDA) content, activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) sampled from the hippocampus, ischemic penumbra or core of cortex were measured. HBO-PC decreased mortality rate, improved neurological recovery, lessened neuronal injury, reduced the level of MDA and increased the antioxidant activity of CAT and SOD. These observations demonstrated that an upregulation of the antioxidant enzyme activity by HBO preconditioning plays an important role in the generation of tolerance against brain ischemia-reperfusion injury. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：223 / 229

页数：7

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