Comparative evaluation of Sensititre YeastOne and VITEK 2 against the Clinical and Laboratory Standards Institute M27-E4 reference broth microdilution method for the antifungal susceptibility testing of Cryptococcus neoformans and Cryptococcus gattii

被引：6
作者：
Zhang, Min [1 ]
Zhou, Ziyi [1 ]
Wang, Dongjiang [1 ]
Zhou, Aiping [1 ]
Song, Guobin [1 ]
Chen, Xingchun [2 ]
Guo, Jian [1 ]
Wu, Wenjuan [1 ]
机构：
[1] Tongji Univ, Shanghai East Hosp, Dept Lab Med, Sch Med, Shanghai 200120, Peoples R China
[2] Guangxi Acad Med Sci, Peoples Hosp Guangxi Zhuang Autonomous Reg, Dept Clin Lab, 6 Taoyuan Rd, Nanning 530021, Guangxi Zhuang, Peoples R China
来源：
MEDICAL MYCOLOGY | 2022年 / 60卷 / 03期
基金：
中国国家自然科学基金;
关键词：
antifungal susceptibility; Cryptococcus; CLSI; Sensititre YeastOne; VITEK; 2; HIGH-DOSE FLUCONAZOLE; AMPHOTERICIN-B; CANDIDA SPP; EPIDEMIOLOGY; FLUCYTOSINE; MENINGITIS; MACRODILUTION; VORICONAZOLE; DISEASE; TRENDS;
D O I：
10.1093/mmy/myac009
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The present study collected 280 isolates of Cryptococcus neoformans and 22 isolates of Cryptococcus gattii and evaluated the consistencies between Sensititre YeastOne (SYO), VITEK 2, and the reference broth microdilution (BMD) method for the antifungal susceptibility testing of fluconazole, voriconazole, and flucytosine. For amphotericin B, SYO was replaced with the BIO KONT amphotericin B microbroth dilution kit. The essential agreements (EAs) by SYO and VITEK 2 for C. neoformans var. grubii and C. neoformans var. neoformans were 98.15-99.63% and 88.89-100%, respectively, with fluconazole, voriconazole, and flucytosine. The EA for C. gattii VG I against fluconazole was 29.41% by VITEK 2, while the other EAs for C. gattii strains were 100% by SYO and VITEK 2. The categorical agreements (CAs) by SYO and VITEK 2 for C. neoformans and C. gattii were 94.12-100%, while VITEK 2 failed to distinguish 5/6 fluconazole-resistant non-wild-type C. neoformans var. grubii isolates; this finding confirmed the limitation of testing fluconazole against C. neoformans as documented in the manufacturer's package insert. The EAs for amphotericin B were 97.42-100% for C. neoformans and C. gattii, but the CAs were 17.65-60% by BIO KONT and VITEK 2. Thus, SYO can be used for susceptibility testing of C. neoformans and C. gattii against fluconazole, voriconazole, and flucytosine, and VITEK 2 can be used for susceptibility testing of C. neoformans against voriconazole and flucytosine. Further studies using amphotericin B-resistant isolates are required to assess the agreement between VITEK 2, BIO KONT, and BMD. Lay Summary Sensititre YeastOne showed a good agreement with the CLSI broth microdilution method for C. neoformans and C. gattii against voriconazole and flucytosine. The limitation regarding fluconazole testing against C. neoformans documented in VITEK 2 manufacturer's package insert was confirmed.
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页数：13
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共 33 条
[1]   In vitro Antifungal Susceptibility Profiles of Cryptococcus neoformans var. grubii and Cryptococcus gattii Clinical Isolates in Guangxi, Southern China [J].
Al-Odaini, Najwa ;
Li, Xiu-ying ;
Li, Bing-kun ;
Chen, Xing-chun ;
Huang, Chun-yang ;
Lv, Chun-ying ;
Pan, Kai-su ;
Zheng, Dong-yan ;
Zheng, Yan-qing ;
Liao, Wan-qing ;
Cao, Cun-wei .
FRONTIERS IN MICROBIOLOGY, 2021, 12
[2]   Comparison of the Etest and microdilution method for antifungal susceptibility testing of Cryptococcus neoformans to four antifungal agents [J].
Aller, AI ;
Martín-Mazuelos, E ;
Gutiérrez, MJ ;
Bernal, S ;
Chávez, N ;
Recio, FJ .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2000, 46 (06) :997-1000
[3]   Molecular epidemiology and antifungal susceptibility profiles of clinical Cryptococcus neoformans/Cryptococcus gattii species complex [J].
Bandalizadeh, Zainab ;
Shokohi, Tahereh ;
Badali, Hamid ;
Abastabar, Mahdi ;
Babamahmoudi, Farhang ;
Davoodi, Lotfolah ;
Mardani, Masoud ;
Javanian, Mostafa ;
Cheraghmakani, Hamed ;
Sepidgar, Ali Asghar ;
Badiee, Parisa ;
Khodavaisy, Sadegh ;
Afshari, Setareh Agha Kuchak ;
Ahmadikia, Kazem ;
Seyedmousavi, Seyedmojtaba .
JOURNAL OF MEDICAL MICROBIOLOGY, 2020, 69 (01) :72-81
[4]   Comparative Evaluation of the Vitek 2 Yeast Susceptibility Test and CLSI Broth Microdilution Reference Method for Testing Antifungal Susceptibility of Invasive Fungal Isolates in Italy: the GISIA3 Study [J].
Borghi, Elisa ;
Iatta, Roberta ;
Sciota, Rita ;
Biassoni, Caterina ;
Cuna, Teresa ;
Montagna, Maria Teresa ;
Morace, Giulia .
JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (09) :3153-3157
[5]   Epidemiology of fungal infections in China [J].
Chen, Min ;
Xu, Yuan ;
Hong, Nan ;
Yang, Yali ;
Lei, Wenzhi ;
Du, Lin ;
Zhao, Jingjun ;
Lei, Xia ;
Xiong, Lin ;
Cai, Langqi ;
Xu, Hui ;
Pan, Weihua ;
Liao, Wanqing .
FRONTIERS OF MEDICINE, 2018, 12 (01) :58-75
[6]   Antifungal susceptibility testing using the E test: Comparison with the broth macrodilution technique [J].
Chen, SCA ;
ODonnell, ML ;
Gordon, S ;
Gilbert, GL .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1996, 37 (02) :265-273
[7]   In vitro antifungal susceptibility profiles and genotypes of 308 clinical and environmental isolates of Cryptococcus neoformans var. grubii and Cryptococcus gattii serotype B from north-western India [J].
Chowdhary, Anuradha ;
Randhawa, Harbans Singh ;
Sundar, Gandhi ;
Kathuria, Shallu ;
Prakash, Anupam ;
Khan, Ziauddin ;
Sun, Sheng ;
Xu, Jianping .
JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 60 (07) :961-967
[8]   Comparison of broth macrodilution, broth microdilution and E-test susceptibility tests of Cryptococcus neoformans for fluconazole [J].
Chryssanthou, E ;
Gronfors, C ;
Khanna, N .
MYCOSES, 1997, 40 (11-12) :423-427
[9]   Comparison of the Vitek 2 Antifungal Susceptibility System with the Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) Broth Microdilution Reference Methods and with the Sensititre YeastOne and Etest Techniques for In Vitro Detection of Antifungal Resistance in Yeast Isolates [J].
Cuenca-Estrella, Manuel ;
Gomez-Lopez, Alicia ;
Alastruey-Izquierdo, Ana ;
Bernal-Martinez, Leticia ;
Cuesta, Isabel ;
Buitrago, Maria J. ;
Rodriguez-Tudela, Juan L. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (05) :1782-1786
[10]   Comparison of MIC Test Strip and Sensititre YeastOne with the CLSI and EUCAST Broth Microdilution Reference Methods for In Vitro Antifungal Susceptibility Testing of Cryptococcus neoformans [J].
Delma, Fatima Zohra ;
Al-Hatmi, Abdullah M. S. ;
Buil, Jochem B. ;
van der Lee, Hein ;
Tehupeiory-Kooreman, Marlou ;
de Hoog, G. Sybren ;
Meletiadis, Joseph ;
Verweij, Paul E. .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2020, 64 (04)
1234