In vitro differentiation of human pluripotent stem cells into the B lineage using OP9-MS5 co-culture

被引：6

作者：

Richardson, Simon E. ^{[1
,2
]}

Ghazanfari, Roshanak ^{[4
]}

Chhetri, Jyoti ^{[3
]}

Enver, Tariq ^{[3
,5
]}

Boiers, Charlotta ^{[4
]}

机构：

[1] Univ Cambridge, Wellcome MRC Cambridge Stem Cell Inst, Cambridge CB2 0AW, England

[2] Univ Cambridge, Jeffrey Cheah Biomed Ctr, Dept Haematol, Cambridge CB2 0AW, England

[3] UCL Canc Inst, 72 Huntley St, London WC1E 6DD, England

[4] Lund Univ, Lund Stem Cell Ctr, Dept Lab Med, Div Mol Hematol, S-22184 Lund, Sweden

[5] Lund Univ, Dept Lab Med, Div Mol Med & Gene Therapy, S-22184 Lund, Sweden

来源：

STAR PROTOCOLS | 2021年 / 2卷 / 02期

基金：

瑞典研究理事会; 英国惠康基金;

关键词：

Cell culture; Cell Differentiation; Stem Cells;

D O I：

10.1016/j.xpro.2021.100420

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro differentiation of human pluripotent stem cells (hPSCs) offers a genetically tractable system to examine the physiology and pathology of human tissue development and differentiation. We have used this approach to model the earliest stages of human B lineage development and characterize potential target cells for the in utero initiation of childhood B acute lymphoblastic leukemia. Herein, we detail critical aspects of the protocol including reagent validation, controls, and examples of surface markers used for analysis and cell sorting. For complete details on the use and execution of this protocol, please refer to Boiers et al. (2018).

引用

页数：20

共 8 条

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