Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis

被引：47
作者：
Ashelford, Kevin [1 ]
Eriksson, Maria E. [2 ]
Allen, Christopher M. [3 ]
D'Amore, Rosalinda [1 ]
Johansson, Mikael [2 ]
Gould, Peter [1 ]
Kay, Suzanne [1 ]
Millar, Andrew J. [4 ]
Hall, Neil [1 ]
Hall, Anthony [1 ]
机构：
[1] Univ Liverpool, Sch Biol Sci, Liverpool L69 7ZB, Merseyside, England
[2] Umea Univ, Umea Plant Sci Ctr, Dept Plant Physiol, SE-90187 Umea, Sweden
[3] Appl Biosyst Inc, Warrington WA3 7QH, Cheshire, England
[4] Univ Edinburgh, Sch Biol Sci, Inst Mol Plant Sci, Edinburgh EH9 3JH, Midlothian, Scotland
来源：
GENOME BIOLOGY | 2011年 / 12卷 / 03期
基金：
英国生物技术与生命科学研究理事会; 瑞典研究理事会; 英国工程与自然科学研究理事会;
关键词：
GENETIC-ANALYSIS; KEY PATHWAYS; THALIANA; IDENTIFICATION; EXPRESSION; PROTEIN; POLYMORPHISMS; TRANSCRIPTION; MUTANTS; ENCODES;
D O I：
10.1186/gb-2011-12-3-r28
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.
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页数：12
相关论文
共 43 条
[1]   Naturally occurring variation in Arabidopsis:: an underexploited resource for plant genetics [J].
Alonso-Blanco, C ;
Koornneef, M .
TRENDS IN PLANT SCIENCE, 2000, 5 (01) :22-29
[2]   The AtNFXL1 gene functions as a signaling component of the type A trichothecene-dependent response [J].
Asano, Tomoya ;
Yasuda, Michiko ;
Nakashita, Hideo ;
Kimura, Makoto ;
Yamaguchi, Kazuo ;
Nishiuchi, Takumi .
PLANT SIGNALING & BEHAVIOR, 2008, 3 (11) :991-992
[3]  
BECHTOLD N, 1993, CR ACAD SCI III-VIE, V316, P1194
[4]   Involvement of GIGANTEA gene in the regulation of the cold stress response in Arabidopsis [J].
Cao, SQ ;
Ye, M ;
Jiang, ST .
PLANT CELL REPORTS, 2005, 24 (11) :683-690
[5]   Common sequence polymorphisms shaping genetic diversity in Arabidopsis thaliana [J].
Clark, Richard M. ;
Schweikert, Gabriele ;
Toomajian, Christopher ;
Ossowski, Stephan ;
Zeller, Georg ;
Shinn, Paul ;
Warthmann, Norman ;
Hu, Tina T. ;
Fu, Glenn ;
Hinds, David A. ;
Chen, Huaming ;
Frazer, Kelly A. ;
Huson, Daniel H. ;
Schoelkopf, Bernhard ;
Nordborg, Magnus ;
Raetsch, Gunnar ;
Ecker, Joseph R. ;
Weigel, Detlef .
SCIENCE, 2007, 317 (5836) :338-342
[6]   Global transcriptome analysis reveals circadian regulation of key pathways in plant growth and development [J].
Covington, Michael F. ;
Maloof, Julin N. ;
Straume, Marty ;
Kay, Steve A. ;
Harmer, Stacey L. .
GENOME BIOLOGY, 2008, 9 (08)
[7]   Identification of MIR390a precursor processing-defective mutants in Arabidopsis by direct genome sequencing [J].
Cuperus, Josh T. ;
Montgomery, Taiowa A. ;
Fahlgren, Noah ;
Burke, Russell T. ;
Townsend, Tiffany ;
Sullivan, Christopher M. ;
Carrington, James C. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (01) :466-471
[8]   Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors:: unprecedented sensitivity reveals novel root- and shoot-specific genes [J].
Czechowski, T ;
Bari, RP ;
Stitt, M ;
Scheible, WR ;
Udvardi, MK .
PLANT JOURNAL, 2004, 38 (02) :366-379
[9]   Variation in crossing-over rates across chromosome 4 of Arabidopsis thaliana reveals the presence of meiotic recombination "hot spots" [J].
Drouaud, J ;
Camilleri, C ;
Bourguignon, PY ;
Canaguier, A ;
Bérard, A ;
Vezon, D ;
Giancola, S ;
Brunel, D ;
Colot, V ;
Prum, B ;
Quesneville, H ;
Mézard, C .
GENOME RESEARCH, 2006, 16 (01) :106-114
[10]   FLOWERING LOCUS C mediates natural variation in the high-temperature response of the Arabidopsis circadian clock [J].
Edwards, KD ;
Anderson, PE ;
Hall, A ;
Salathia, NS ;
Locke, JCW ;
Lynn, JR ;
Straume, M ;
Smith, JQ ;
Millar, AJ .
PLANT CELL, 2006, 18 (03) :639-650
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