Myoglobin as a New Fluorescence Probe to Sense H2S

被引：37

作者：

Strianese, Maria ^{[1
]}

De Martino, Franco ^{[1
]}

Pellecchia, Claudio ^{[1
]}

Ruggiero, Giuseppe ^{[2
]}

D'Auria, Sabato ^{[2
]}

机构：

[1] Univ Salerno, Dept Chem, I-84084 Fisciano, Sa, Italy

[2] CNR, Lab Mol Sensing, IBP, I-80131 Naples, Italy

来源：

PROTEIN AND PEPTIDE LETTERS | 2011年 / 18卷 / 03期

关键词：

Fluorescence; biosensors; FRET; heme-proteins; Sol-gel; TYPE-3 COPPER PROTEINS; HYDROGEN-SULFIDE; SULFHEME PROTEINS; SULFMYOGLOBIN; MOLECULES; ROLES; AIR;

D O I：

10.2174/092986611794578378

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new, fast, simple and cost-effective sensing device for monitoring H2S has been developed. Proof-of-principle results showing that a commercial and cheap Myoglobin (Mb) can be successfully used as a biological probe for a fluorescence biosensor for H2S detection are reported. Two different commercial labels Cy3 and Atto620 were selected for this study. A high selectivity for detecting H2S against other thiols was found. The applicability of the proposed sensing system was successfully explored not only in solution but also when applied in the form of a solid state device.

引用

页码：282 / 286

页数：5

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