The Myeloid U937 Skin Sensitization Test (U-SENS) addresses the activation of dendritic cell event in the adverse outcome pathway for skin sensitization

被引：191

作者：

Piroird, Cecile ^{[1
]}

Ovigne, Jean-Marc ^{[1
]}

Rousset, Francoise ^{[1
]}

Martinozzi-Teissier, Silvia ^{[1
]}

Gomes, Charles ^{[1
]}

Cotovio, Jose ^{[1
]}

Alepee, Nathalie ^{[1
]}

机构：

[1] LOreal Res & Innovat, F-93600 Aulnay Sous Bois, France

来源：

TOXICOLOGY IN VITRO | 2015年 / 29卷 / 05期

关键词：

Skin sensitization; Dendritic cells; CD86; induction; MUSST; U-SENS (TM); Predictivity; LYMPH-NODE ASSAY; TEST H-CLAT; IN-VITRO; PEPTIDE REACTIVITY; CONTACT ALLERGENS; RISK-ASSESSMENT; END-POINTS; PREDICTION; POTENCY; LINE;

D O I：

10.1016/j.tiv.2015.03.009

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The U-SENS(TM) assay, formerly known as MUSST (Myeloid U937 Skin Sensitization Test), is an in vitro method to assess skin sensitization. Dendritic cell activation following exposure to sensitizers was modelled in the U937 human myeloid cell line by measuring the induction of the expression of CD86 by flow cytometry. The predictive performance of U-SENS(TM) was assessed via a comprehensive comparison analysis with the available human and LLNA data of 175 substances. U-SENS(TM) showed 79% specificity, 90% sensitivity and 88% accuracy. A four laboratory ring study demonstrated the transferability, reliability and reproducibility of U-SENS(TM), with a reproducibility of 95% within laboratories and 79% between-laboratories, showing that the U-SENS(TM) assay is a promising tool in a skin sensitization risk assessment testing strategy. (C) 2015 Published by Elsevier Ltd.

引用

页码：901 / 916

页数：16

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