Anti-allergy Effect of Marc from Silver Vine (Actinidia polygama) Prepared by Supercritical Fluid Extraction

Anti-allergic effect of marc extract after supercritical fluid extraction (SFE) comparing the crude extract from silver vine was investigated to increase its potential for use as a new source of functional food that reduced non-favorable flavor. The results tested by thiazolyl blue tetrazolium bromide (MIT) assay indicated that the ethanol extract of silver vine (ETOH) and ethanol extract of SFE marc (SFEM) showed no cytotoxic effects on IgE-sensitized rat basophilic leukemia (RBL-2H3) cells under the conditions. ETOH, as well as SFEM inhibited antigen-induced degranulation in RBL-2H3 mast cells dose dependently. The morphological change of the cells treated with compound 48/80 was prevented by the extracts. Production of tumor necrosis factor (TNF)-alpha on the activated RBL-2H3 cells was also reduced by ETOH and SFEM, similarly. Moreover, the total phenolic content of the extracts seems to correlate with the anti-allergic activity. In conclusion, the results suggest that the flavor-improved SFE marc extract suppressed the antigen-induced allergic reactions in RBL-2H3 mast cells.