Optical sectioning using single-plane-illumination Raman imaging

被引：18
作者：
Barman, Ishan [1 ]
Tan, Khay Ming [2 ]
Singh, Gajendra Pratap [2 ]
机构：
[1] MIT, George R Harrison Spect Lab, Cambridge, MA 02139 USA
[2] Univ St Andrews, Sch Phys & Astron, St Andrews KY16 9SS, Fife, Scotland
来源：
JOURNAL OF RAMAN SPECTROSCOPY | 2010年 / 41卷 / 10期
关键词：
single-plane-illumination microscopy (SPIM); single-plane-illumination Raman imaging (SPIRI); Raman imaging; Raman spectroscopy; optical sectioning;
D O I：
10.1002/jrs.2785
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
Confocal Raman imaging is widely used for optical sectioning of materials. However, for biological applications it often suffers from poor axial resolution, photodamage to the sample of interest, substrate interference, and long acquisition times. We have applied the principles of light sheet microscopy to Raman imaging and show for the first time that optically sectioned Raman images can be obtained in significantly lower acquisition times. Copyright (C) 2010 John Wiley & Sons, Ltd.
引用
收藏
页码：1099 / 1101
页数：3
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