Highly selective detection of Escherichia coli O157:H7 based on micro-gapped interdigitated electrode arrays

被引：12

作者：

Li, Le ^{[1
]}

Chen, Zhaochangchi ^{[2
]}

Wang, Shujuan ^{[3
]}

Jin, Xin ^{[1
]}

Yang, Lixia ^{[4
]}

Liu, Guangyao ^{[5
]}

Zhao, Jinfeng ^{[1
]}

机构：

[1] Cent S Univ, Xiangya Hosp, Chinese Minist Hlth, Key Lab Nanobiol Technol, Changsha, Hunan, Peoples R China

[2] Xinxiang Med Univ, Dept Clin Med, Xinxiang, Peoples R China

[3] Univ Shanghai Sci & Technol, Sch Med Instrument & Food Engn, Ctr Syst Biomed Sci, Shanghai, Peoples R China

[4] Changsha Inst Food & Drug Control, Dept Sci Res, Changsha, Hunan, Peoples R China

[5] Hunan Yuantai Biotechnol Co Ltd, Changsha, Hunan, Peoples R China

来源：

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT | 2017年 / 31卷 / 05期

关键词：

Escherichia coli O157:H7; micro-gapped interdigitated electrode array; immunoassay; electrochemical sensing; POLYMERASE-CHAIN-REACTION; SIGNAL AMPLIFICATION; SALMONELLA-ENTERICA; SENSITIVE DETECTION; IMMUNOSENSOR; ENRICHMENT; PATHOGENS; OUTBREAKS; PROTEINS; SAMPLES;

D O I：

10.1080/13102818.2017.1335178

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Development of effective pathogen detection systems is critical for human health. In this study, a micro-gapped interdigitated electrode array-based immunosensor chip is constructed for the detection of a typical pathogen, Escherichia coli (E. coli) O157:H7. The immunosesnor chip was prepared via photolithography and characterized by a digital camera, optical microscope and scanning electron microscope. The E. coli O157:H7 detection mechanism is based on an enzyme-linked, sandwiched immunoassay with enzymatic silver deposition. Our results revealed that the proposed immunosensor chip showed selective electrical response towards E. coli O157:H7 over other bacteria including Hemolytic streptococcus, Staphylococcus aureus, Salmonella, Pseudomonas aeruginosa, Chronobacter sakazakii and Bacillus cereus. Our assay exhibited potential practical applications for E. coli O157:H7 determination in water and our methodology might be applied to detect different pathogens by simply selecting specific antibodies.

引用

页码：1070 / 1078

页数：9

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