TNF-α inhibits UCP-1 expression in brown adipocytes via ERKs -: Opposite effect of p38MAPK

被引：56
作者：
Valladares, A
Roncero, C
Benito, M
Porras, A
机构：
[1] CSIC, Ctr Mixto, Inst Bioquim, Dept Bioquim & Biol Mol 2, Madrid 28040, Spain
[2] Univ Complutense Madrid, Fac Farm, E-28040 Madrid, Spain
来源：
FEBS LETTERS | 2001年 / 493卷 / 01期
关键词：
brown adipocyte; tumor necrosis factor-alpha; uncoupling protein-1; p38 mitogen-activated protein kinase; extracellular-regulated kinase;
D O I：
10.1016/S0014-5793(01)02264-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Tumor necrosis factor-alpha (TNF-alpha) activates extracellular-regulated kinases (ERKs) and p38 mitogen-activated protein kinase (p38MAPK), and inhibits the expression of uncoupling protein-1 (UCP-1) and adipocyte-specific genes in rat fetal brown adipocytes. MEK inhibition with PD98059 abolished the inhibitory effect of TNF-alpha on UCP-1, but not on adipogenic genes. In contrast, inhibition of p38MAPK with SB203580 potentiated the negative effect of TNF-alpha on UCP-1 and adipogenic genes. The inhibitory action of TNF-alpha was partially correlated with changes in C/EBP alpha and beta protein levels and in their DNA binding activity suggesting a role for these transcription factors, However, other transcription factors might explain the different regulation of UCP-1 and adipogenic genes by ERKs, (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
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页码：6 / 11
页数：6
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