Peptide TQS169 prevents osteoporosis in rats by enhancing osteogenic differentiation and calcium absorption

被引：17
作者：
Wang, Huan [1 ]
Chen, Nanye [1 ]
Shen, Shuru [1 ]
Li, Huiyu [1 ]
Hu, Xiangxiang [1 ]
Yang, Yunkai [1 ]
Yu, Xiaochen [1 ]
Ye, Li [1 ]
Zhou, Wei [2 ]
Feng, Meiqing [1 ]
机构：
[1] Fudan Univ, Sch Pharm, Dept Microbiol & Biochem Pharm, Shanghai 201203, Peoples R China
[2] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China
来源：
JOURNAL OF FUNCTIONAL FOODS | 2018年 / 49卷
关键词：
Low-calcium diet; MC3T3-E1; cell; Ossotide; Osteogenic differentiation; Osteoporosis; MARROW STROMAL CELLS; GENE-EXPRESSION; POSTMENOPAUSAL WOMEN; HUMAN OSTEOBLASTS; RETINOIC ACID; BONE LOSS; PROLIFERATION; DEFICIENCY; GROWTH; MODEL;
D O I：
10.1016/j.jff.2018.08.021
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The preventive effects of TQS169 peptide on osteoporosis were investigated and the mechanisms of promoting osteogenic differentiation and calcium absorption were also studied in vitro and vivo in this research. Wistar rats were given a low-Calcium diet treated with different doses of peptide TQS169 and extra CaCl2 for 4 weeks. The content of Calcium, OPG in serum and the Ca content of the femur were increased in high-dose-treated rats. The osteoporosis model induced by retinoic acid was established to measure the related factors. High dosage of TQS169 treated group showed the protective effects. Osteoblastogenesis differentiation of MC3T3-E1 cells treated with TQS169 were accelerated through improving ALP activity, promoting mineral matrix formation and the expression of typical genes of RUNX2, OCN and COL I. Taking all the experiments together, our study confirms the protective effects of TQS169 on osteoporosis and explains the potential mechanism for the first time.
引用
收藏
页码：113 / 121
页数：9
相关论文
共 35 条
  • [1] RETRACTED: Tridax procumbens flavonoids: a prospective bioactive compound increased osteoblast differentiation and trabecular bone formation (Retracted Article)
    Al Mamun, Md. Abdullah
    Hosen, Mohammad Jakir
    Khatun, Amina
    Alam, M. Masihul
    Al-Bari, Md. Abdul Alim
    [J]. BIOLOGICAL RESEARCH, 2017, 50
  • [2] Bone strength and its determinants
    Ammann, P
    Rizzoli, R
    [J]. OSTEOPOROSIS INTERNATIONAL, 2003, 14 (Suppl 3) : S13 - S18
  • [3] Milk ribonuclease-enriched lactoferrin induces positive effects on bone turnover markers in postmenopausal women
    Bharadwaj, S.
    Naidu, A. G. T.
    Betageri, G. V.
    Prasadarao, N. V.
    Naidu, A. S.
    [J]. OSTEOPOROSIS INTERNATIONAL, 2009, 20 (09) : 1603 - 1611
  • [4] High calcium, phosphate and calcitriol supplementation leads to an osteocyte-like phenotype in calcified vessels and bone mineralisation defect in uremic rats
    Bisson, Sarah-Kim
    Ung, Roth-Visal
    Picard, Sylvain
    Valade, Danika
    Agharazii, Mohsen
    Lariviere, Richard
    Mac-Way, Fabrice
    [J]. JOURNAL OF BONE AND MINERAL METABOLISM, 2019, 37 (02) : 212 - 223
  • [5] Watching osteogenesis: Life monitoring of osteogenic differentiation using an osteocalcin reporter
    Born, Anne-Kathrin
    Lischer, Stefanie
    Maniura-Weber, Katharina
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 2012, 113 (01) : 313 - 321
  • [6] MANAGEMENT OF ENDOCRINE DISEASE Novel anabolic treatments for osteoporosis
    Canalis, Ernesto
    [J]. EUROPEAN JOURNAL OF ENDOCRINOLOGY, 2018, 178 (02) : R33 - R44
  • [7] Hesperidin, a citrus flavonoid, inhibits bone loss and decreases serum and hepatic lipids in ovariectomized mice
    Chiba, H
    Uehara, M
    Wu, J
    Wang, XX
    Masuyama, R
    Suzuki, K
    Kanazawa, K
    Ishimi, Y
    [J]. JOURNAL OF NUTRITION, 2003, 133 (06) : 1892 - 1897
  • [8] Effect of dietary calcium deficiency and altered diet hardness on the jawbone growth: A micro-CT and bone histomorphometric study in rats
    Fujita, Yuko
    Goto, Shota
    Ichikawa, Maika
    Hamaguchi, Ayako
    Maki, Kenshi
    [J]. ARCHIVES OF ORAL BIOLOGY, 2016, 72 : 200 - 210
  • [9] The bone-specific expression of Runx2 oscillates during the cell cycle to support a G1-related antiproliferative function in osteoblasts
    Galindo, M
    Pratap, J
    Young, DW
    Hovhannisyan, H
    Im, HJ
    Choi, JY
    Lian, JB
    Stein, JL
    Stein, GS
    van Wijnen, AJ
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (21) : 20274 - 20285
  • [10] Hodgson Stephen F, 2003, Endocr Pract, V9, P544
1234