A highly adhesive and naturally derived sealant

被引：218

作者：

Assmann, Alexander ^{[1
,2
,3
,4
,5
]}

Vegh, Andrea ^{[1
,2
,6
]}

Ghasemi-Rad, Mohammad ^{[1
,2
]}

Bagherifard, Sara ^{[1
,2
,7
]}

Cheng, George ^{[8
,9
]}

Sani, Ehsan Shirzaei ^{[10
]}

Ruiz-Esparza, Guillermo U. ^{[1
,2
]}

Noshadi, Iman ^{[1
,2
,3
,10
]}

Lassaletta, Antonio D. ^{[9
]}

Gangadharan, Sidhu ^{[9
]}

Tamayol, Ali ^{[1
,2
,3
]}

Khademhosseini, Ali ^{[1
,2
,3
,11
,12
]}

Annabi, Nasim ^{[1
,2
,3
,10
]}

机构：

[1] Harvard Med Sch, Brigham & Womens Hosp, Biomat Innovat Res Ctr, 65 Landsdowne St, Cambridge, MA 02139 USA

[2] MIT, Harvard MIT Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[3] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[4] Heinrich Heine Univ, Med Fac, Dept Cardiovasc Surg, D-40225 Dusseldorf, Germany

[5] Heinrich Heine Univ, Med Fac, Res Grp Expt Surg, D-40225 Dusseldorf, Germany

[6] Univ Toronto, Dept Mat Sci & Engn, Toronto, ON M5S 1A4, Canada

[7] Politecn Milan, Dept Mech Engn, I-20156 Milan, Italy

[8] Duke Univ, Med Ctr, Div Pulm Allergy & Crit Care, Durham, NC 27710 USA

[9] Beth Israel Deaconess Med Ctr, Div Thorac Surg & Intervent Pulmonol, Boston, MA 02215 USA

[10] Northeastern Univ, Dept Chem Engn, Boston, MA 02115 USA

[11] King Abdulaziz Univ, Dept Phys, Jeddah 21569, Saudi Arabia

[12] Konkuk Univ, Coll Anim Biosci & Technol, Dept Bioind Technol, Seoul 05029, South Korea

来源：

BIOMATERIALS | 2017年 / 140卷

基金：

美国国家卫生研究院;

关键词：

Gelatin methacryloyl (GelMA); Hydrogel; Lung lesion; Sealant; Wound repair; GELATIN METHACRYLATE HYDROGELS; INTRAOPERATIVE AIR LEAKS; TISSUE ADHESIVE; LUNG SURGERY; BIOMEDICAL APPLICATIONS; PULMONARY LOBECTOMY; POLYMERIC SEALANT; SURGICAL SEALANT; FIBRIN SEALANT; WOUND CLOSURE;

D O I：

10.1016/j.biomaterials.2017.06.004

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Conventional surgical techniques to seal and repair defects in highly stressed elastic tissues are insufficient. Therefore, this study aimed to engineer an inexpensive, highly adhesive, biocompatible, and biodegradable sealant based on a modified and naturally derived biopolymer, gelatin methacryloyl (GelMA). We tuned the degree of gelatin modification, prepolymer concentration, photoinitiator concentration, and crosslinking conditions to optimize the physical properties and adhesion of the photo-crosslinked GelMA sealants. Following ASTM standard tests that target wound closure strength, shear resistance, and burst pressure, GelMA sealant was shown to exhibit adhesive properties that were superior to clinically used fibrin- and poly(ethylene glycol)-based glues. Chronic in vivo experiments in small as well as translational large animal models proved GelMA to effectively seal large lung leakages without the need for sutures or staples, presenting improved performance as compared to fibrin glue, poly(ethylene glycol) glue and sutures only. Furthermore, high biocompatibility of GelMA sealant was observed, as evidenced by a low inflammatory host response and fast in vivo degradation while allowing for adequate wound healing at the same time. Combining these results with the low costs, ease of synthesis and application of the material, GelMA sealant is envisioned to be commercialized not only as a sealant to stop air leakages, but also as a biocompatible and biodegradable hydrogel to support lung tissue regeneration. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：115 / 127

页数：13

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