Long-Term, Noninvasive In Vivo Tracking of Progenitor Cells Using Multimodality Photoacoustic, Optical Coherence Tomography, and Fluorescence Imaging

被引：32

作者：

Van Phuc Nguyen ^{[1
]}

Fan, Wen ^{[1
,2
]}

Zhu, Tianye ^{[1
,2
]}

Qian, Wei ^{[3
]}

Li, Yanxiu ^{[1
]}

Liu, Bing ^{[3
]}

Zhang, Wei ^{[4
]}

Henry, Jessica ^{[1
]}

Yuan, Songtao ^{[2
]}

Wang, Xueding ^{[4
]}

Paulus, Yannis M. ^{[1
,4
]}

机构：

[1] Univ Michigan, Dept Ophthalmol & Visual Sci, Ann Arbor, MI 48105 USA

[2] Nanjing Med Univ, Dept Ophthalmol, Affiliated Hosp 1, Nanjing 210029, Peoples R China

[3] IMRA Amer Inc, Ann Arbor, MI 48105 USA

[4] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48105 USA

来源：

ACS NANO | 2021年 / 15卷 / 08期

关键词：

stem cells; regenerative medicine; photoacoustic microscopy; optical coherence tomography; contrast agents; progenitor human retinal pigment epithelium cells; gold nanoparticles; MESENCHYMAL STEM-CELLS; MICROSCOPY;

D O I：

10.1021/acsnano.1c03035

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Stem cell regenerative medicine therapies have emerged as promising treatments for currently incurable diseases. A remaining challenge for cell therapies is the ability to track the migration and distribution of the transplanted cells in a long-term, noninvasive manner in vivo to assess their efficacy. This study develops a noninvasive, and high spatial resolution photoacoustic microscopy (PAM) and optical coherence tomography (OCT) imaging system for in vivo tracking of subretinally injected progenitor human retinal pigment epithelium cells (ARPE-19) labeled with chainlike gold nanoparticle (CGNP) clusters in RPE damage. CGNP provided significant PAM, OCT, and fluorescence signals to selectively track the migration of ARPE-19 cells in living rabbit eyes for 3 months. PAM and OCT imaging allow accurate anatomical information to determine the exact retinal layer in which the transplanted ARPE-19 cells are located which was confirmed by histology. This presents an efficient and advanced technology to visualize fundamental biological processes of cell therapies in complex in vivo environments in real time.

引用

页码：13289 / 13306

页数：18

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